High stretchability, strength, and toughness of living cells enabled by hyperelastic vimentin intermediate filaments

被引：113

作者：

Hu, Jiliang ^{[1
]}

Li, Yiwei ^{[1
]}

Hao, Yukun ^{[1
]}

Zheng, Tianqi ^{[1
]}

Gupta, Satish K. ^{[1
]}

Parada, German Alberto ^{[2
]}

Wu, Huayin ^{[3
]}

Lin, Shaoting ^{[1
]}

Wang, Shida ^{[1
]}

Zhao, Xuanhe ^{[1
,4
]}

Goldman, Robert D. ^{[5
]}

Cai, Shengqiang ^{[6
]}

Guo, Ming ^{[1
]}

机构：

[1] MIT, Dept Mech Engn, Cambridge, MA 02139 USA

[2] MIT, Dept Chem Engn, Cambridge, MA 02139 USA

[3] Harvard Univ, Sch Engn & Appl Sci, Cambridge, MA 02138 USA

[4] MIT, Dept Civil & Environm Engn, Cambridge, MA 02139 USA

[5] Northwestern Univ, Feinberg Sch Med, Dept Cell & Mol Biol, Chicago, IL 60611 USA

[6] Univ Calif San Diego, Dept Mech & Aerosp Engn, La Jolla, CA 92093 USA

来源：

PROCEEDINGS OF THE NATIONAL ACADEMY OF SCIENCES OF THE UNITED STATES OF AMERICA | 2019年 / 116卷 / 35期

基金：

美国国家卫生研究院;

关键词：

vimentin; intermediate filament; cytoskeleton; cytoplasm; cell mechanics; MECHANICS; CYTOPLASM; MIGRATION; DYNAMICS; BEHAVIOR; FIBERS; REPAIR;

D O I：

10.1073/pnas.1903890116

中图分类号：

O [数理科学和化学]; P [天文学、地球科学]; Q [生物科学]; N [自然科学总论];

学科分类号：

07 ; 0710 ; 09 ;

摘要：

In many developmental and pathological processes, including cellular migration during normal development and invasion in cancer metastasis, cells are required to withstand severe deformations. The structural integrity of eukaryotic cells under small deformations has been known to depend on the cytoskeleton including actin filaments ( F-actin), microtubules ( MT), and intermediate filaments ( IFs). However, it remains unclear how cells resist severe deformations since both F-actin and microtubules yield or disassemble under moderate strains. Using vimentin containing IFs ( VIFs) as a model for studying the large family of IF proteins, we demonstrate that they dominate cytoplasmic mechanics and maintain cell viability at large deformations. Our results show that cytoskeletal VIFs form a stretchable, hyperelastic network in living cells. This network works synergistically with other cytoplasmic components, substantially enhancing the strength, stretchability, resilience, and toughness of cells. Moreover, we find the hyperelastic VIF network, together with other quickly recoverable cytoskeletal components, forms a mechanically robust structure which can mechanically recover after damage.

引用

页码：17175 / 17180

页数：6

共 41 条

[11] Vimentin Intermediate Filaments Template Microtubule Networks to Enhance Persistence in Cell Polarity and Directed Migration [J].