Protoporphyrin IX distribution in rat brain following administration of 5-aminolevulinic acid or its hexylester

被引：0

作者：

Madsen, SJ ^{[1
]}

Friesen, SA ^{[1
]}

Hjortland, GO ^{[1
]}

Engebraaten, O ^{[1
]}

Peng, Q ^{[1
]}

Hirschberg, H ^{[1
]}

机构：

[1] Univ Nevada, Dept Hlth Phys, Las Vegas, NV 89154 USA

来源：

LASERS IN SURGERY: ADVANCED CHARACTERIZATION, THERAPEUTICS, AND SYSTEMS XIV | 2004年 / 5312卷

关键词：

5-aminolevulinic acid; ALA derivatives; ALA hexyl ester; protoporphyrin IX; photodynamic therapy; brain tumor model; glioblastoma multiforme;

D O I：

10.1117/12.527791

中图分类号：

R-3 [医学研究方法]; R3 [基础医学];

学科分类号：

1001 ;

摘要：

The distribution of protoporphyrin IX (Pp IX) was investigated in a novel animal model following administration of 5-aminolevulinic acid (ALA) or its hexyl ester (h-ALA). Pre-cultured tumor spheroids prepared from cell lines or tumor biopsy fragments were injected into the brains of immunodeficient rats. Approximately 3 months after spheroid implantation, Rowett nude rats were injected intra-cranially with the maximum tolerable ALA or h-ALA dose. Animals were sacrificed 4 hours post-injection and their brains removed for fluorescence microscopy analysis. The primary finding of this study is that, in the tumor biopsy model, the tumor-to-normal brain PpIX fluorescence ratio is approximately 3 times higher following direct in situ h-ALA administration compared to ALA.

引用

页码：396 / 404

页数：9

共 18 条

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