Phyto-saponins as a natural adjuvant for delivery of agromaterials through plant cuticle membranes

被引：27

作者：

Chapagain, Bishnu P. ^{[1
]}

Wiesman, Zeev ^{[1
]}

机构：

[1] Ben Gurion Univ Negev, Inst Appl Res, Dept Biotechnol Engn, Phytolipid Biotechnol Lab, IL-84105 Beer Sheva, Israel

来源：

JOURNAL OF AGRICULTURAL AND FOOD CHEMISTRY | 2006年 / 54卷 / 17期

关键词：

phyto-saponins; cuticle membrane; phytoadjuvant; nanovesicles; Balanites aegyptiaca; Quillaja saponaria;

D O I：

10.1021/jf060591y

中图分类号：

S [农业科学];

学科分类号：

09 ;

摘要：

With growing use of synthetic adjuvants in modern agriculture, their impacts on the environment are being questioned. In a search for an environmentally safe phyto-adjuvant, we have investigated natural glycosidic saponin for delivery of agromaterials through plant cuticle membranes. Four saponin preparations from Quillaja saponaria bark (QE), obtained from Sigma-Aldrich, and Balanites aegyptiaca fruit mesocarp (ME), kernel (KE), and root (RE), isolated and characterized in our laboratory, were used for testing the delivery of [C-14]-2,4-dichlorophenoxyacetic acid (2,4-D) across isolated intact astomatous adaxial Citrus grandis leaf cuticle membranes (CMs). The results showed that both Q. saponaria and B. aegyptiaca saponin preparations enhanced delivery of 2,4-D through CMs. Among the saponin preparations, ME exhibited a significantly higher level of delivery of 2,4-D with a concentration effect (2% being the highest). Transmission electron microscope (TEM) and dynamic light scattering (DLS) characterization of these saponin preparations in aqueous solution clearly demonstrated the formation of nanoscale vesicles. Various possibilities for a natural amphiphatic phyto-saponin as a delivery adjuvant through CMs are discussed.

引用

页码：6277 / 6285

页数：9

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