Photoactivatable synthetic Ras proteins:: "Baits" for the identification of plasma-membrane-bound binding partners of Ras

被引：0

作者：

Kuhlmann, J

Tebbe, A

Völkert, M

Wagner, M

Uwai, K

Waldmann, H

机构：

[1] Max Planck Inst Mol Physiol, Abt Strukturelle Biol, D-44227 Dortmund, Germany

[2] Max Planck Inst Mol Physiol, Abt Chem Biol, D-44227 Dortmund, Germany

[3] Univ Dortmund, Fb 3, D-44227 Dortmund, Germany

来源：

ANGEWANDTE CHEMIE-INTERNATIONAL EDITION | 2002年 / 41卷 / 14期

关键词：

lipoproteins; photoactivation; protein engineering; signal transduction;

D O I：

10.1002/1521-3773(20020715)41:14<2546::AID-ANIE2546>3.0.CO;2-E

中图分类号：

O6 [化学];

学科分类号：

0703 ;

摘要：

The combination of organic synthesis with cell biology provides access to a chimera of the signal-transducing Ras protein, which contains a photoactivatable benzophenone group in its isoprenoid membrane anchor, yet retains biological activity. The semisynthetic protein is a new tool to address open questions concerning the mechanism of selective plasma membrane localization of Ras and to identify putative binding partners of Ras.

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页码：2546 / +

页数：6

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