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Detection of telomerase activity by the.TRAP assay and its variants and alternatives
被引:60
作者:
Fajkus, Jiri
[1
]
机构:
[1] Masaryk Univ, Fac Sci, Dept Funct Genom & Proteom, CS-61137 Brno, Czech Republic
[2] Acad Sci Czech Republ, Inst Biophys, CZ-61265 Brno, Czech Republic
关键词:
telomerase;
TRAP;
PCR;
real-time;
in situ;
biosensor;
D O I:
10.1016/j.cca.2006.02.039
中图分类号:
R446 [实验室诊断];
R-33 [实验医学、医学实验];
学科分类号:
1001 ;
摘要:
Telomerase activity is closely connected to problems of cellular immortality, proliferative capacity, differentiation, cancer and aging. Correspondingly, techniques for its detection have been essential for progress in telomere biology and are of still increasing importance in molecular diagnostics and therapy of cancer. This article reviews the development of the telomere repeat amplification protocol (TRAP) and its various modifications as the most widespread assay to detect and measure telomerase activity. Alternative possibilities of telomerase activity detection are also discussed which make it possible to omit the PCR-mediated amplification of telomerase products. These approaches are based on recent advances in highly sensitive detection systems. (c) 2006 Elsevier B.V. All rights reserved.
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页码:25 / 31
页数:7
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