Ftx is dispensable for imprinted X-chromosome inactivation in preimplantation mouse embryos

被引：27

作者：

Soma, Miki ^{[1
]}

Fujihara, Yoshitaka ^{[2
]}

Okabe, Masaru ^{[2
]}

Ishino, Fumitoshi ^{[1
]}

Kobayashi, Shin ^{[1
,3
]}

机构：

[1] Tokyo Med & Dent Univ, Med Res Inst, Dept Epigenet, Bunkyo Ku, Tokyo 1138510, Japan

[2] Osaka Univ, Res Inst Microbial Dis, Suita, Osaka 5650871, Japan

[3] Japan Sci & Technol Agcy, PRESTO, Kawaguchi, Saitama 3320012, Japan

来源：

SCIENTIFIC REPORTS | 2014年 / 4卷

基金：

日本科学技术振兴机构;

关键词：

XIST EXPRESSION; NONCODING RNA; DOSAGE; GENE; MICE;

D O I：

10.1038/srep05181

中图分类号：

O [数理科学和化学]; P [天文学、地球科学]; Q [生物科学]; N [自然科学总论];

学科分类号：

07 ; 0710 ; 09 ;

摘要：

X-chromosome inactivation (XCI) equalizes gene expression between the sexes by inactivating one of the two X chromosomes in female mammals. Xist has been considered as a major cis-acting factor that inactivates the paternally derived X chromosome (Xp) in preimplantation mouse embryos (imprinted XCI). Ftx has been proposed as a positive regulator of Xist. However, the physiological role of Ftx in female animals has never been studied. We recently reported that Ftx is located in the cis-acting regulatory region of the imprinted XCI and expressed from the inactive Xp, suggesting a role in the imprinted XCI mechanism. Here we examined the effects on imprinted XCI using targeted deletion of Ftx. Disruption of Ftx did not affect the survival of female embryos or expression of Xist and other X-linked genes in the preimplantation female embryos. Our results indicate that Ftx is dispensable for imprinted XCI in preimplantation embryos.

引用

页数：6

共 20 条

[1] Ftx is a non-coding RNA which affects Xist expression and chromatin structure within the X-inactivation center region [J].

Chureau, Corinne ;

Chantalat, Sophie ;

Romito, Antonio ;

Galvani, Angelique ;

Duret, Laurent ;

Avner, Philip ;

Rougeulle, Claire .

HUMAN MOLECULAR GENETICS, 2011, 20 (04) :705-718

[2] Inheritance of a pre-inactivated paternal X chromosome in early mouse embryos [J].

Huynh, KD ;

Lee, JT .

NATURE, 2003, 426 (6968) :857-862

[3] IMPRINTING AND X-CHROMOSOME COUNTING MECHANISMS DETERMINE XIST EXPRESSION IN EARLY MOUSE DEVELOPMENT [J].

KAY, GF ;

BARTON, SC ;

SURANI, MA ;

RASTAN, S .

CELL, 1994, 77 (05) :639-650

[4] Comparison of gene expression in male and female mouse blastocysts revealed imprinting of the X-linked gene, Rhox5/Pem, at preimplantation stages [J].

Kobayashi, S ;

Isotani, A ;

Mise, N ;

Yamamoto, M ;

Fujihara, Y ;

Kaseda, K ;

Nakanishi, T ;

Ikawa, M ;

Hamada, H ;

Abe, K ;

Okabe, M .

CURRENT BIOLOGY, 2006, 16 (02) :166-172

[5] Identification of an Imprinted Gene Cluster in the X-Inactivation Center [J].

Kobayashi, Shin ;

Totoki, Yasushi ;

Soma, Miki ;

Matsumoto, Kazuya ;

Fujihara, Yoshitaka ;

Toyoda, Atsushi ;

Sakaki, Yoshiyuki ;

Okabe, Masaru ;

Ishino, Fumitoshi .

PLOS ONE, 2013, 8 (08)

[6] The X-linked imprinted gene family Fthl17 shows predominantly female expression following the two-cell stage in mouse embryos [J].

Kobayashi, Shin ;

Fujihara, Yoshitaka ;

Mise, Nathan ;

Kaseda, Kazuhiro ;

Abe, Kuniya ;

Ishino, Fumitoshi ;

Okabe, Masaru .

NUCLEIC ACIDS RESEARCH, 2010, 38 (11) :3672-3681

[7] Disruption of imprinted X inactivation by parent-of-origin effects at Tsix [J].

Lee, JT .

CELL, 2000, 103 (01) :17-27

[8] Reactivation of the paternal X chromosome in early mouse embryos [J].

Mak, W ;

Nesterova, TB ;

de Napoles, M ;

Appanah, R ;

Yamanaka, S ;

Otte, AP ;

Brockdorff, N .

SCIENCE, 2004, 303 (5658) :666-669

[9] Xist-deficient mice are defective in dosage compensation but not spermatogenesis [J].

Marahrens, Y ;

Panning, B ;

Dausman, J ;

Strauss, W ;

Jaenisch, R .

GENES & DEVELOPMENT, 1997, 11 (02) :156-166

[10] FISH analysis of 142 EGFP transgene integration sites into the mouse genome [J].

Nakanishi, T ;

Kuroiwa, A ;

Yamada, S ;

Isotani, A ;

Yamashita, A ;

Tairaka, A ;

Hayashi, T ;

Takagi, T ;

Ikawa, M ;

Matsuda, Y ;

Okabe, M .

GENOMICS, 2002, 80 (06) :564-574

← 1 2 →