A simplified method for the HPLC resolution of α-carotene and β-carotene (trans and cis) isomers

被引：13

作者：

Bononi, M ^{[1
]}

Commissati, I ^{[1
]}

Lubian, E ^{[1
]}

Fossati, A ^{[1
]}

Tateo, F ^{[1
]}

机构：

[1] Univ Milan, Lab Anal Alimenti, Di Pro Ve FAc Agr, I-20133 Milan, Italy

来源：

ANALYTICAL AND BIOANALYTICAL CHEMISTRY | 2002年 / 372卷 / 02期

关键词：

HPLC; carotene isomers;

D O I：

10.1007/s00216-001-1144-3

中图分类号：

Q5 [生物化学];

学科分类号：

071010 ; 081704 ;

摘要：

A new HPLC/DAD (Diode Array Detector) method is proposed for the identification of some carotene isomers. The operating conditions adopted permit the resolution of alpha-carotene, all-trans-beta-carotene, 9-cis-beta-carotene, 13-cis-beta-carotene and 15-cis-beta-carotene. Moreover, the chromatographic conditions reported are simplified in respect of those reported up to now. The method is applied to the determination of carotenoids in a dried Dunaliella salina extract, but it could be also applied to other organic matrices such as eggs.

引用

页码：401 / 403

页数：3

共 21 条

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