MiRNA-93-5p promotes the biological progression of gastric cancer cells via Hippo signaling pathway

被引：5

作者：

Meng, H. ^{[1
]}

Li, Y-Y ^{[1
]}

Han, D. ^{[1
]}

Zhang, C-Y ^{[1
]}

机构：

[1] Jining Med Coll, Huxi Affiliated Hosp, Shanxian Cent Hosp Shangdong Prov, Dept Gastroenterol, Jining, Shanxian County, Peoples R China

来源：

EUROPEAN REVIEW FOR MEDICAL AND PHARMACOLOGICAL SCIENCES | 2019年 / 23卷 / 11期

关键词：

MiRNA-93-5p; Hippo Pathway; Gastric cancer; Biological functions; PROLIFERATION; MIR-93; IDENTIFICATION; DEREGULATION; BIOMARKERS; INVASION; MIRNAS; TARGET; GROWTH;

D O I：

暂无

中图分类号：

R9 [药学];

学科分类号：

1007 ;

摘要：

OBJECTIVE: To clarify the influence of microRNA-93-5p (miRNA-93-5p) on biological behaviors of gastric cancer (GC) cells and its regulatory effect on Hippo pathway. MATERIALS AND METHODS: SGC-7901 and HGC-27 cells were used for establishing miRNA-93-5p overexpression and downregulation model through transfection of miRNA-93-5p mimics or inhibitor, respectively. Relative levels of genes in Hippo pathway were determined in GC cells transfected with miRNA-93-5p mimics or inhibitor by quantitative Real-time polymerase chain reaction (qRT-PCR). Regulatory effects of miRNA-93-5p on proliferative, migratory and invasive abilities of GC cells were evaluated by cell counting kit-8 (CCK-8), colony formation and transwell assay, respectively. RESULTS: MiRNA-93-5p was markedly upregulated by transfection of miRNA-93-5p mimics into SGC-7901 cells, which was downregulated by transfection of miRNA-93-5p inhibitor into HGC-27 cells. Overexpression of miRNA-93-5p accelerated GC cells to proliferate, migrate and invade. Meanwhile, miRNA-93-5p overexpression in GC cells upregulated downstream genes in Hippo pathway, including CDX2, FOXM1 and CTGF. CONCLUSIONS: MiRNA-93-5p enhances proliferative, migratory and invasive abilities of GC cells by activating Hippo pathway, which may serve as a diagnostic and therapeutic target for GC.

引用

页码：4763 / 4769

页数：7

共 34 条

[31] Targeting YAP-Dependent MDSC Infiltration Impairs Tumor Progression [J].

Wang, Guocan ;

Lu, Xin ;

Dey, Prasenjit ;

Deng, Pingna ;

Wu, Chia Chin ;

Jiang, Shan ;

Fang, Zhuangna ;

Zhao, Kuo ;

Konaparthi, Ramakrishna ;

Hua, Sujun ;

Zhang, Jianhua ;

Li-Ning-Tapia, Elsa M. ;

Kapoor, Avnish ;

Wu, Chang-Jiun ;

Patel, Neelay Bhaskar ;

Guo, Zhenglin ;

Ramamoorthy, Vandhana ;

Tieu, Trang N. ;

Heffernan, Tim ;

Zhao, Di ;

Shang, Xiaoying ;

Khadka, Sunada ;

Hou, Pingping ;

Hu, Baoli ;

Jin, Eun-Jung ;

Yao, Wantong ;

Pan, Xiaolu ;

Ding, Zhihu ;

Shi, Yanxia ;

Li, Liren ;

Chang, Qing ;

Troncoso, Patricia ;

Logethetis, Christopher J. ;

McArthur, Mark J. ;

Chin, Lynda ;

Wang, Y. Alan ;

DePinho, Ronaldo A. .

CANCER DISCOVERY, 2016, 6 (01) :80-95

[32] Exosomal miR-93 promotes proliferation and invasion in hepatocellular carcinoma by directly inhibiting TIMP2/TP53INP1/CDKN1A [J].

Xue, Xiaofeng ;

Wang, Xiaona ;

Zhao, Yubin ;

Hu, Rongkuan ;

Qin, Lei .

BIOCHEMICAL AND BIOPHYSICAL RESEARCH COMMUNICATIONS, 2018, 502 (04) :515-521

[33] Long non-coding RNA PTENP1 functions as a ceRNA to modulate PTEN level by decoying miR-106b and miR-93 in gastric cancer [J].

Zhang, Rupeng ;

Guo, Yuenan ;

Ma, Zhenchi ;

Ma, Gang ;

Xue, Qiang ;

Li, Fangxuan ;

Liu, Liren .

ONCOTARGET, 2017, 8 (16) :26079-26089

[34]

Zygulska AL, 2017, J PHYSIOL PHARMACOL, V68, P311

← 1 2 3 4 →