Systematic Identification of Methyl Jasmonate-Responsive Long Noncoding RNAs and Their Nearby Coding Genes Unveils Their Potential Defence Roles in Tobacco BY-2 Cells

被引：8

作者：

Zheng, Kaifeng ^{[1
]}

Wang, Zitao ^{[2
,3
]}

Pang, Lu ^{[1
]}

Song, Zhongbang ^{[4
]}

Zhao, Heping ^{[1
]}

Wang, Yingdian ^{[1
,3
]}

Wang, Bingwu ^{[4
]}

Han, Shengcheng ^{[1
,3
]}

机构：

[1] Beijing Normal Univ, Coll Life Sci, Beijing Key Lab Gene Resources & Mol Dev, Beijing 100875, Peoples R China

[2] Qinghai Normal Univ, Coll Life Sci, Xining 810008, Peoples R China

[3] Qinghai Normal Univ, Beijing Normal Univ, Acad Plateau Sci & Sustainabil Peoples Govt Qingha, Xining 810008, Peoples R China

[4] Yunnan Acad Tobacco Agr Sci, Kunming 650021, Peoples R China

来源：

INTERNATIONAL JOURNAL OF MOLECULAR SCIENCES | 2022年 / 23卷 / 24期

基金：

中国国家自然科学基金;

关键词：

long noncoding RNA; methyl jasmonate; tobacco; nicotine synthesis; plant defence; ALKALOID BIOSYNTHESIS; TRANSCRIPTION FACTORS; 9-LIPOXYGENASE PATHWAY; NICOTINE BIOSYNTHESIS; REGULATORS; EXPRESSION; ROOT; METABOLISM; REVEALS; ENOD40;

D O I：

10.3390/ijms232415568

中图分类号：

Q5 [生物化学]; Q7 [分子生物学];

学科分类号：

071010 ; 081704 ;

摘要：

Long noncoding RNAs (lncRNAs) are distributed in various species and play critical roles in plant growth, development, and defence against stimuli. However, the lncRNA response to methyl jasmonate (MeJA) treatment has not been well characterized in Nicotiana tabacum Bright Yellow-2 (BY-2) cells, and their roles in plant defence remain elusive. Here, 7848 reliably expressed lncRNAs were identified in BY-2 cells, of which 629 differentially expressed (DE) lncRNAs were characterized as MeJA-responsive lncRNAs. The lncRNAs in BY-2 cells had a strong genus specificity in Nicotiana. The combined analysis of the cis-regulated lncRNAs and their target genes revealed the potential up- and downregulated target genes that are responsible for different biological functions and metabolic patterns. In addition, some lncRNAs for response-associated target genes might be involved in plant defence and stress resistance via their MeJA- and defence-related cis-regulatory elements. Moreover, some MeJA-responsive lncRNA target genes were related to quinolinate phosphoribosyltransferase, lipoxygenases, and endopeptidase inhibitors, which may contribute to nicotine synthesis and disease and insect resistance, indicating that MeJA-responsive lncRNAs regulate nicotine biosynthesis and disease resistance by regulating their potential target genes in BY-2 cells. Therefore, our results provide more targets for genetically engineering the nicotine content and plant defence in tobacco plants.

引用

页数：16