Purification and characterization of bacteriocin F1, a novel bacteriocin produced by Lactobacillus paracasei subsp tolerans FX-6 from Tibetan kefir, a traditional fermented milk from Tibet, China

被引：85

作者：

Miao, Jianyin ^{[1
,2
]}

Guo, Haoxian ^{[1
]}

Ou, Yangwen ^{[3
]}

Liu, Guo ^{[1
]}

Fang, Xiang ^{[1
]}

Liao, Zhenlin ^{[1
]}

Ke, Chang ^{[1
]}

Chen, Yunjiao ^{[1
]}

Zhao, Lichao ^{[1
]}

Cao, Yong ^{[1
]}

机构：

[1] South China Agr Univ, Coll Food Sci, Guangzhou 510542, Guangdong, Peoples R China

[2] Qinzhou Univ, Sch Ocean, Qinzhou 535000, Peoples R China

[3] Hunan Univ Chinese Med, Sch Pharm, Changsha 410000, Hunan, Peoples R China

来源：

FOOD CONTROL | 2014年 / 42卷

关键词：

Lactobacillus paracasei subsp tolerans; Bacteriocin; Purification characterization; Tibet kefir; ANTIMICROBIAL PEPTIDES; RESISTANCE; CABBAGE; CULTURE; GRAINS; LACTIS; FOOD;

D O I：

10.1016/j.foodcont.2014.01.041

中图分类号：

TS2 [食品工业];

学科分类号：

0832 ;

摘要：

A bacteriocin-producing lactic acid bacteria, FX-6, was isolated from Tibetan kefir using the agar well diffusion assay. Strain FX-6 was identified as Lactobacillus paracasei subsp. tolerans on the basis of 16S rDNA sequence analyses. This bacteriocin, which was designated bacteriocin Fl, was purified by a threestep purification procedure. The molecular mass of bacteriocin Fl was 2113.842 Da by MALDI-TOF MS analyses. It was also discovered to have blocked N-termini, hampering analysis by Edman degradation. Bacteriocin Fl exhibited a wide range of antimicrobial activity, strong heat stability (20minat 121 C, or 60 min at 100 degrees C) and pH stability (pH 3.0-9.0). Following treatment by pepsin and trypsin, the antibacterial activity was partly reduced. Bacteriocin Fl is the first reported bacteriocin produed by L paracasei subsp. tolerans which can not only inhibit fungi but also bacteria. The characterization of bacteriocin Fl suggested that it was a novel bacteriocin with potential research and application value in food industry. (C) 2014 Elsevier Ltd. All rights reserved.

引用

页码：48 / 53

页数：6

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