β- Conglycinin Reduces the Tight Junction Occludin and ZO-1 Expression in IPEC-J2

被引：38
作者：
Zhao, Yuan [1 ,2 ]
Qin, Guixin [1 ,2 ]
Han, Rui [1 ]
Wang, Jun [1 ]
Zhang, Xiaodong [3 ]
Liu, Dandan [1 ]
机构：
[1] Jilin Agr Univ, Coll Anim Sci & Technol, Changchun 130118, Peoples R China
[2] Jilin Agr Univ, Minist Educ, Key Lab Anim Prod Prod Qual & Secur, Changchun 130118, Peoples R China
[3] Jilin Univ, Coll Vet Med, Key Lab Zoonosis Res, Inst Zoonosis,Minist Educ, Changchun 130062, Peoples R China
来源：
INTERNATIONAL JOURNAL OF MOLECULAR SCIENCES | 2014年 / 15卷 / 02期
基金：
中国国家自然科学基金;
关键词：
-conglycinin; intestinal barrier; tight junction; food allergen; IPEC-J2; INTESTINAL ALKALINE-PHOSPHATASE; TRANSEPITHELIAL ANTIGEN TRANSPORT; EARLY-WEANED PIG; FOOD ALLERGY; IN-VITRO; SOYBEAN-MEAL; PERMEABILITY; MODEL; HYPERSENSITIVITY; CELLS;
D O I：
10.3390/ijms15021915
中图分类号：
Q5 [生物化学]; Q7 [分子生物学];
学科分类号：
071010 ; 081704 ;
摘要：
Soybean allergy presents a health threat to humans and animals. The mechanism by which food/feed allergen -conglycinin injures the intestinal barrier has not been well understood. In this study, the changes of epithelial permeability, integrity, metabolic activity, the tight junction (TJ) distribution and expression induced by -conglycinin were evaluated using IPEC-J2 model. The results showed a significant decrease of trans-epithelial electrical resistance (TEER) (p < 0.001) and metabolic activity (p < 0.001) and a remarkable increase of alkaline phosphatase (AP) activity (p < 0.001) in a dose-dependent manner. The expression levels of tight junction occludin and ZO-1 were decreased (p < 0.05). The reduced fluorescence of targets and change of cellular morphology were recorded. The tight junction occludin and ZO-1 mRNA expression linearly declined with increasing -conglycinin (p < 0.001).
引用
收藏
页码：1915 / 1926
页数：12
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