Microsatellite DNA markers for Plasmopara viticola, the causal agent of downy mildew of grapes

被引：48
|
作者：
Delmotte, F.
Chen, W. J.
Richard-Cervera, S.
Greif, C.
Papura, D.
Giresse, X.
Mondor-Genson, G.
Corio-Costet, M. F.
机构：
[1] INRA, UMR Sante Vegetale 1065, ENITA, Ctr Rech Bordeaux Aquitaine, Villenave Dornon, France
[2] ULP, INRA, UMR Sante Vigne & Qual Vin, F-68021 Colmar, France
[3] INRA, Ctr Rech Colmar, Colman, France
[4] CBGP, F-34988 Montferrier Sur Lez, France
来源：
MOLECULAR ECOLOGY NOTES | 2006年 / 6卷 / 02期
关键词：
enriched library; grape downy mildew; microsatellite; Plasmopara viticola;
D O I：
10.1111/j.1471-8286.2005.01240.x
中图分类号：
Q5 [生物化学]; Q7 [分子生物学];
学科分类号：
071010 ; 081704 ;
摘要：
Microsatellite loci were isolated from Plasmopara viticola (Oomycetes), the causal agent of downy mildew of grape, one of the most damaging fungal diseases of grapevine worldwide. Seven polymorphic loci were obtained from an enriched partial genomic library. A low genetic diversity was observed at all loci, with a mean observed allele number of 3.75 and an observed heterozygosity ranging from 0.074 to 0.547. Cross-amplification tests on three closely related taxa indicated that two loci could be used in other Oomycetes species. These microsatellite loci were proved to be useful for population genetic analysis.
引用
收藏
页码：379 / 381
页数：3
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