Stromal Cell-Derived Factor-I (SDF-1)/CXCR4 Axis Enhances Cellular Invasion in Ovarian Carcinoma Cells Via Integrin β1 and β3 Expressions

被引：11

作者：

Yu, Yuecheng ^{[1
]}

Shi, Xiaoyan ^{[2
]}

Shu, Zhen ^{[1
]}

Xie, Tingting ^{[1
]}

Huang, Kan ^{[1
]}

Wei, Li ^{[1
]}

Song, Hui ^{[1
]}

Zhang, Wei ^{[3
]}

Xue, Xiaochang ^{[3
]}

机构：

[1] Fourth Mil Med Univ, Xijing Hosp, Dept Obstet & Gynecol, Xian 710032, Peoples R China

[2] Yanan Univ, Affiliated Hosp, Dept Obstet & Gynecol, Yaan, Peoples R China

[3] Fourth Mil Med Univ, Sch Pharm, Ctr Biotechnol, State Key Lab Canc Biol, Xian 710032, Peoples R China

来源：

ONCOLOGY RESEARCH | 2013年 / 21卷 / 04期

关键词：

Ovarian cancer; Invasion; Migration; Metastasis; Integrin; BREAST-CANCER CELLS; LIPID RAFTS; METASTASIS; GROWTH; CXCR4; INVOLVEMENT; RECEPTORS; SYSTEM;

D O I：

10.3727/096504014X13907540404879

中图分类号：

R73 [肿瘤学];

学科分类号：

100214 ;

摘要：

Accumulating evidence has showed that strornal cell-derived factor-1 (SDF-1/CXCR4) axis played important roles in cancer metastases, but the detailed function in ovarian cancer is still largely unknown. In the present study, we determined the location of CXCR4 and lipid rafts, a specialized structure on cell membrane, in ovarian cancer tissues and ovarian cancer cell line SKOV3 cells by immunaluorescence. To analyze the role of SDF-1/CXCR4 and lipid rafts in tumor cell migration and invasion, Transwell assay and wound healing assay were also performed. Cytoflowmetry was carried out to determine the participation of integrins. Our data showed that CXCR4 and GM1 (marker of lipid rafts) were expressed in both ovarian cancer tissue and SKOV3 cells, and SDF-1 promoted the invasion and migration of SKOV3 cells, which was mediated by complete lipid rafts. Further studies uncovered that SDF-1 upregulated the expression of integrin beta 1 and beta 3, two molecules closely related with cancer metastasis. These results indicated that SDF-1 might promote the invasion and metastasis of ovarian cancer by regulating these two integrin molecules.

引用

页码：217 / 225

页数：9

共 35 条

[31]

Van Slambrouck S, 2007, INT J ONCOL, V31, P1501

[32] β-Catenin is a promising key factor in the SDF-1/CXCR4 axis on metastasis of pancreatic cancer [J].

Wang, Zheng ;

Ma, Qjngyong .

MEDICAL HYPOTHESES, 2007, 69 (04) :816-820

[33] Growth factors in human ovarian cancer [J].

Westermann, AM ;

Beijnen, JH ;

Moolenaar, WH ;

Rodenhuis, S .

CANCER TREATMENT REVIEWS, 1997, 23 (02) :113-131

[34] CXCR4/SDF-1 pathway is crucial for TLR9 agonist enhanced metastasis of human lung cancer cell [J].

Xu, Lin ;

Zhou, Ya ;

Liu, Qi ;

Luo, Jun-Min ;

Qing, Min ;

Tang, Xian-Yin ;

Yao, Xin-Shen ;

Wang, Chun-Hong ;

Wen, Zhen-Ke .

BIOCHEMICAL AND BIOPHYSICAL RESEARCH COMMUNICATIONS, 2009, 382 (03) :571-576

[35] Function of the chemokine receptor CXCR4 in haematopoiesis and in cerebellar development [J].

Zou, YR ;

Kottmann, AH ;

Kuroda, M ;

Taniuchi, I ;

Littman, DR .

NATURE, 1998, 393 (6685) :595-599

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