RNA interference: Biology and prospects of application in biomedicine and biotechnology

被引：13

作者：

Vilgelm, A. E. ^{[1
]}

Chumakov, S. P. ^{[1
]}

Prassolov, V. S. ^{[1
]}

机构：

[1] Russian Acad Sci, VA Engelhardt Mol Biol Inst, Moscow 119991, Russia

来源：

MOLECULAR BIOLOGY | 2006年 / 40卷 / 03期

基金：

俄罗斯基础研究基金会;

关键词：

gene expression regulation; posttranscriptional regulation of gene expression; RNA interference; small interfering RNA; miRNA;

D O I：

10.1134/S0026893306030010

中图分类号：

Q5 [生物化学]; Q7 [分子生物学];

学科分类号：

071010 ; 081704 ;

摘要：

RNA interference is one of the most important mechanisms regulating gene expression. Small interfering RNAs (siRNAs) play an essential role in cell defense against virus infection or retrotransposons. The use of siRNAs gives wide opportunities for treating virus infections and cancer. RNA interference allows rapid construction of monogenic functional knockouts, thereby providing a convenient tool for researchers. The review considers the current views of the mechanisms of RNA interference and modern approaches to its practical application.

引用

页码：339 / 354

页数：16

共 99 条

[21] Processing of primary microRNAs by the Microprocessor complex [J].

Denli, AM ;

Tops, BBJ ;

Plasterk, RHA ;

Ketting, RF ;

Hannon, GJ .

NATURE, 2004, 432 (7014) :231-235

[22] RNA interference in mammalian cells using siRNAs synthesized with T7 RNA polymerase -: art. no. e46 [J].

Donzé, O ;

Picard, D .

NUCLEIC ACIDS RESEARCH, 2002, 30 (10) :e46

[23] The silent revolution: RNA interference as basic biology, research tool, and therapeutic [J].

Dykxhoorn, DM ;

Lieberman, J .

ANNUAL REVIEW OF MEDICINE, 2005, 56 :401-423

[24] RNA interference is mediated by 21-and 22-nucleotide RNAs [J].

Elbashir, SM ;

Lendeckel, W ;

Tuschl, T .

GENES & DEVELOPMENT, 2001, 15 (02) :188-200

[25] Analysis of gene function in somatic mammalian cells using small interfering RNAs [J].

Elbashir, SM ;

Harborth, J ;

Weber, K ;

Tuschl, T .

METHODS, 2002, 26 (02) :199-213

[26] Systemic silencing signal(s) [J].

Fagard, M ;

Vaucheret, H .

PLANT MOLECULAR BIOLOGY, 2000, 43 (2-3) :285-293

[27] Potent and specific genetic interference by double-stranded RNA in Caenorhabditis elegans [J].

Fire, A ;

Xu, SQ ;

Montgomery, MK ;

Kostas, SA ;

Driver, SE ;

Mello, CC .

NATURE, 1998, 391 (6669) :806-811

[28] INVOLVEMENT OF THE CD95 (APO-1/FAS) RECEPTOR AND LIGAND IN LIVER-DAMAGE [J].

GALLE, PR ;

HOFMANN, WJ ;

WALCZAK, H ;

SCHALLER, H ;

OTTO, G ;

STREMMEL, W ;

KRAMMER, PH ;

RUNKELL, L .

JOURNAL OF EXPERIMENTAL MEDICINE, 1995, 182 (05) :1223-1230

[29] Small interfering RNA inhibits hepatitis B virus replication in mice [J].

Giladi, H ;

Ketzinel-Gilad, M ;

Rivkin, L ;

Felig, Y ;

Nussbaum, O ;

Galun, E .

MOLECULAR THERAPY, 2003, 8 (05) :769-776

[30] Micro-RNAs: small is plentiful [J].

Grosshans, H ;

Slack, FJ .

JOURNAL OF CELL BIOLOGY, 2002, 156 (01) :17-21

← 1 2 3 4 5 6 7 8 9 10 →