MICROSATELLITE MARKERS FOR IDENTIFICATION OF A GROUP OF ITALIAN OLIVE ACCESSIONS

被引:23
作者
Muzzalupo, Innocenzo [1 ,2 ]
Stefanizzi, Francesca [1 ]
Salimonti, Amelia [1 ]
Falabella, Rosanna [1 ]
Perri, Enzo [1 ]
机构
[1] Consiglio Ric & Sperimentaz Agr, Ctr Ric Olivicoltura & Ind CRA OLI, I-87036 Arcavacata Di Rende, CS, Italy
[2] Consiglio Ric & Sperimentaz Agr, Ctr Ric Prod Foraggere & Lattiero Casearie CRA FL, I-26900 Lodi, Italy
关键词
Olea europaea; fingerprinting; genetic identity; molecular markers; OLEA-EUROPAEA L; SEQUENCE REPEATS SSRS; GENETIC DIVERSITY; DNA; POLYMORPHISM; POPULATIONS; LOCUS; WILD;
D O I
10.1590/S0103-90162009000500014
中图分类号
S [农业科学];
学科分类号
09 ;
摘要
Cultivar characterization for fruit trees certification requires fast, efficient and reliable techniques. Microsatellite markers (SSR) were used in the molecular characterization of 23 genotypes of Olea europaea subsp europaea. The DNA from the olive cultivars was analyzed using nine preselected SSR primers (GAPU59, GAPU71A, GAPU71B, GAPU103A, UDO99-01, UDO99-12, UDO99-28 and UDO99-39) and revealed 29 alleles, which allowed each genotype to be identified. In the dendrogram, the nine primers allowed the 23 olive genotypes to be grouped into subgroups corresponding to the same cultivar denominations. SSR markers proved to be efficient and reliable for the molecular characterization of Italian olive cultivars.
引用
收藏
页码:685 / 690
页数:6
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