Direct reprogramming of human neural stem cells by OCT4

被引：488

作者：

Kim, Jeong Beom ^{[1
]}

Greber, Boris ^{[1
]}

Arauzo-Bravo, Marcos J. ^{[1
]}

Meyer, Johann ^{[2
]}

Park, Kook In ^{[3
]}

Zaehres, Holm ^{[1
]}

Schoeler, Hans R. ^{[1
]}

机构：

[1] Max Planck Inst Mol Biomed, Dept Cell & Dev Biol, D-48149 Munster, NRW, Germany

[2] Hannover Med Sch, Dept Expt Hematol, D-30625 Hannover, Germany

[3] Yonsei Univ, Coll Med, Dept Pediat, Project Med Sci BK21, Seoul 120752, South Korea

来源：

NATURE | 2009年 / 461卷 / 7264期

关键词：

HUMAN FIBROBLASTS; GENERATION; MOUSE; PLURIPOTENCY; INDUCTION; MYC;

D O I：

10.1038/nature08436

中图分类号：

O [数理科学和化学]; P [天文学、地球科学]; Q [生物科学]; N [自然科学总论];

学科分类号：

07 ; 0710 ; 09 ;

摘要：

Induced pluripotent stem (iPS) cells have been generated from mouse and human somatic cells by ectopic expression of four transcription factors (OCT4 (also called POU5F1), SOX2, c-Myc and KLF4)(1-7). We previously reported that Oct4 alone is sufficient to reprogram directly adult mouse neural stem cells to iPS cells(8). Here we report the generation of one-factor human iPS cells from human fetal neural stem cells (one-factor (1F) human NiPS cells) by ectopic expression of OCT4 alone. One-factor human NiPS cells resemble human embryonic stem cells in global gene expression profiles, epigenetic status, as well as pluripotency in vitro and in vivo. These findings demonstrate that the transcription factor OCT4 is sufficient to reprogram human neural stem cells to pluripotency. One-factor iPS cell generation will advance the field further towards understanding reprogramming and generating patient-specific pluripotent stem cells.

引用

页码：649 / U93

页数：6

共 25 条

[1] Efficient and rapid generation of induced pluripotent stem cells from human keratinocytes [J].

Aasen, Trond ;

Raya, Angel ;

Barrero, Maria J. ;

Garreta, Elena ;

Consiglio, Antonella ;

Gonzalez, Federico ;

Vassena, Rita ;

Bilic, Josipa ;

Pekarik, Vladimir ;

Tiscornia, Gustavo ;

Edel, Michael ;

Boue, Stephanie ;

Izpisua Belmonte, Juan Carlos .

NATURE BIOTECHNOLOGY, 2008, 26 (11) :1276-1284

[2] Generation of pluripotent stem cells from adult mouse liver and stomach cells [J].

Aoi, Takashi ;

Yae, Kojiro ;

Nakagawa, Masato ;

Ichisaka, Tomoko ;

Okita, Keisuke ;

Takahashi, Kazutoshi ;

Chiba, Tsutomu ;

Yamanaka, Shinya .

SCIENCE, 2008, 321 (5889) :699-702

[3] Induction of pluripotent stem cells from primary human fibroblasts with only Oct4 and Sox2 [J].

Danwei Huangfu ;

Osafune, Kenji ;

Maehr, Rene ;

Guo, Wenjun ;

Eijkelenboom, Astrid ;

Chen, Shuibing ;

Muhlestein, Whitney ;

Melton, Douglas A. .

NATURE BIOTECHNOLOGY, 2008, 26 (11) :1269-1275

[4] Induced pluripotent stem cells generated from patients with ALS can be differentiated into motor neurons [J].

Dimos, John T. ;

Rodolfa, Kit T. ;

Niakan, Kathy K. ;

Weisenthal, Laurin M. ;

Mitsumoto, Hiroshi ;

Chung, Wendy ;

Croft, Gist F. ;

Saphier, Genevieve ;

Leibel, Rudy ;

Goland, Robin ;

Wichterle, Hynek ;

Henderson, Christopher E. ;

Eggan, Kevin .

SCIENCE, 2008, 321 (5893) :1218-1221

[5] Generation of Human Induced Pluripotent Stem Cells by Direct Delivery of Reprogramming Proteins [J].

Kim, Dohoon ;

Kim, Chun-Hyung ;

Moon, Jung-Il ;

Chung, Young-Gie ;

Chang, Mi-Yoon ;

Han, Baek-Soo ;

Ko, Sanghyeok ;

Yang, Eungi ;

Cha, Kwang Yul ;

Lanza, Robert ;

Kim, Kwang-Soo .

CELL STEM CELL, 2009, 4 (06) :472-476

[6] Pluripotent stem cells induced from adult neural stem cells by reprogramming with two factors [J].

Kim, Jeong Beom ;

Zaehres, Holm ;

Wu, Guangming ;

Gentile, Luca ;

Ko, Kinarm ;

Sebastiano, Vittorio ;

Arauzo-Bravo, Marcos J. ;

Ruau, David ;

Han, Dong Wook ;

Zenke, Martin ;

Schoeler, Hans R. .

NATURE, 2008, 454 (7204) :646-U54

[7] Oct4-Induced Pluripotency in Adult Neural Stem Cells [J].

Kim, Jeong Beom ;

Sebastiano, Vittorio ;

Wu, Guangming ;

Arauzo-Bravo, Marcos J. ;

Sasse, Philipp ;

Gentile, Luca ;

Ko, Kinarm ;

Ruau, David ;

Ehrich, Mathias ;

van den Boom, Dirk ;

Meyer, Johann ;

Huebner, Karin ;

Bernemann, Christof ;

Ortmeier, Claudia ;

Zenke, Martin ;

Fleischmann, Bernd K. ;

Zaehres, Holm ;

Schoeler, Hans R. .

CELL, 2009, 136 (03) :411-419

[8] Human neurospheres derived from the fetal central nervous system are regionally and temporally specified but are not committed [J].

Kim, Young-Tai ;

Kim, Il-Sun ;

Lee, Il-Shin ;

Lee, Jean-Pyo ;

Snyder, Evan Y. ;

Park, Kook In .

EXPERIMENTAL NEUROLOGY, 2006, 199 (01) :222-235

[9] Generation of human induced pluripotent stem cells from dermal fibroblasts [J].

Lowry, W. E. ;

Richter, L. ;

Yachechko, R. ;

Pyle, A. D. ;

Tchieu, J. ;

Sridharan, R. ;

Clark, A. T. ;

Plath, K. .

PROCEEDINGS OF THE NATIONAL ACADEMY OF SCIENCES OF THE UNITED STATES OF AMERICA, 2008, 105 (08) :2883-2888

[10] Directly reprogrammed fibroblasts show global epigenetic remodeling and widespread tissue contribution [J].

Maherali, Nimet ;

Sridharan, Rupa ;

Xie, Wei ;

Utikal, Jochen ;

Eminli, Sarah ;

Arnold, Katrin ;

Stadtfeld, Matthias ;

Yachechko, Robin ;

Tchieu, Jason ;

Jaenisch, Rudolf ;

Plath, Kathrin ;

Hochedlinger, Konrad .

CELL STEM CELL, 2007, 1 (01) :55-70

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