Effect of Simmental bull seminal plasma protein in egg yolk-citrate extender on Kacang buck semen fertility

被引:18
作者
Susilowati, Suherni [1 ,4 ]
Triana, Indah Norma [2 ]
Sardjito, Trilas [1 ]
Suprayogi, Tri Wahyu [1 ]
Wurlina, Wurlina [2 ]
Mustofa, Imam [3 ]
机构
[1] Lab Vet Artificial Inseminat, Surabaya, Indonesia
[2] Lab Vet Infertil & Steril, Surabaya, Indonesia
[3] Lab Vet Obstet, Surabaya, Indonesia
[4] Univ Airlangga, Dept Vet Reprod, Fac Vet Med, Kampus C Unair, Surabaya, Indonesia
关键词
DNA fragmentation; Intact plasma membrane; Malondialdehyde; Motility; Viability; SPERM DNA-DAMAGE; ARTIFICIAL-INSEMINATION; MOLECULAR MARKERS; QUALITY; CRYOPRESERVATION; SYNCHRONIZATION; SPERMATOZOA; GOATS; PROTAMINE; PROFILE;
D O I
10.1016/j.cryobiol.2020.10.013
中图分类号
Q [生物科学];
学科分类号
07 ; 0710 ; 09 ;
摘要
The genetic resources of Indonesia's indigenous Kacang goat require preservation. Artificial insemination is expected to accelerate population increases and preserve genetic resources simultaneously. The present study was the maiden attempt for cryopreservation of Kacang buck sperm. The objectives of this study were to determine whether the supplementation of superior Simmental bull seminal plasma protein in egg yolk-citrate extender could improve the quality of post-thawed Kacang buck sperm, increase conceptions rates, and improve kidding rates. Buck semen was diluted without supplementation (T0) and with supplementation of 2.5 mg (T1) and 5 mg (T2) of Simmental bull seminal plasma protein per mL egg yolk-citrate extender. Extended semen was packed in 0.25 mL straw as cryopreserved frozen semen. Post-thawed semen samples were evaluated for viability, motility, intact plasma membranes, malondialdehyde level, and DNA fragmentation. Estrus was synchronized for sixty Kacang does, which were divided randomly into three groups and inseminated using post-thawed semen. The progesterone serum concentration of the does was measured 7 and 22 days post-insemination to detect ovulation and conception. Pregnancy was confirmed using abdominal palpation at 43 days post-insemination and by observing birth. The T1 group showed the highest (P < 0.05) post-thawed viability, motility, and intact plasma membrane. Conception, pregnancy and kidding rates were also higher in T1 than other treatment groups. In conclusion, the 2.5 mg Simmental bull seminal plasma protein supplementation per mL egg yolk-citrate extender provided the best seminal quality and fertility of post-thawed Kacang buck semen.
引用
收藏
页码:20 / 27
页数:8
相关论文
共 21 条
[11]   Cholesterol-cyclodextrin complex as a replacement for egg yolk in bull semen extender: sperm characteristics post-thawing and in vivo fertility [J].
Yang, Steve X. ;
Adams, Gregg P. ;
Zwiefelhofer, Eric M. ;
Rajapaksha, Kosala ;
Anzar, Muhammad .
ANIMAL REPRODUCTION SCIENCE, 2021, 225
[12]   Effect of seminal plasma and egg yolk concentration on freezability of goat semen [J].
Ferreira, Valeria da Silva ;
Bourg de Mello, Marco Roberto ;
Moreira da Fonseca, Carlos Elysio ;
Ferreira Dias, Allan Cesar ;
Cardoso, Jessica Machado ;
Silva, Rebecca Barbosa ;
Martins Junior, Wagner Pereira .
REVISTA BRASILEIRA DE ZOOTECNIA-BRAZILIAN JOURNAL OF ANIMAL SCIENCE, 2014, 43 (10) :513-518
[13]   EFFECT OF LACTOSE EGG YOLK GLYCEROL EXTENDER SUPPLEMENTED WITH TREHALOSE ON POST-THAW CHARACTERISTICS AND FERTILITY OF BUFFALO BULL SPERMATOZOA [J].
Mughal, Dawar Hameed ;
Ahamd, Ijaz ;
Yousaf, Muhammad Shehbaz ;
Farooq, Umer ;
Wadood, Fazal ;
Riaz, Amjad .
SCIENTIFIC PAPERS-SERIES D-ANIMAL SCIENCE, 2021, 64 (02) :180-186
[14]   Effect of the type of egg yolk, removal of seminal plasma and donor age on buck sperm cryopreservation [J].
Tabarez, Abigail ;
Garcia, Wilber ;
Jesus Palomo, Maria .
SMALL RUMINANT RESEARCH, 2017, 149 :91-98
[15]   In Vitro Evaluation of Liquid-stored Buffalo Semen at 5°C Diluted in Soya Lecithin Based Extender (Bioxcell®), Tris-Citric Egg Yolk, Skim Milk and Egg Yolk-Citrate Extenders [J].
Akhter, S. ;
Ansari, M. S. ;
Rakha, B. A. ;
Ullah, N. ;
Andrabi, S. M. H. ;
Khalid, M. .
REPRODUCTION IN DOMESTIC ANIMALS, 2011, 46 (01) :45-49
[16]   Effect of Addition of Different Concentrations of Alpha Lipoic Acid to Tris Egg Yolk Citrate Glycerol Extender on Cryopreservation of Sahiwal Bull Spermatozoa [J].
Ahmad, Syed Bilal ;
Ahmad, Ijaz ;
Ahmad, Nazir ;
Qureshi, Zafar Iqbal ;
Jamil, Huma ;
Ali, Qaisar ;
Ashfaq, Khurram .
PAKISTAN VETERINARY JOURNAL, 2018, 38 (03) :301-305
[17]   Effect of Egg Yolk and Soybean Lecithin on Tris-Based Extender in Post-Thaw Ram Semen Quality and in vitro Fertility [J].
Ustuner, Burcu ;
Alcay, Selim ;
Nur, Zekariya ;
Sagirkaya, Hakan ;
Soylu, M. Kemal .
KAFKAS UNIVERSITESI VETERINER FAKULTESI DERGISI, 2014, 20 (03) :393-398
[18]   Effect of rainbow trout (Oncorhynchus mykiss) seminal plasma on the post-thaw quality of ram semen cryopreserved in a soybean lecithin-based or egg yolk-based extender [J].
Ustuner, Burcu ;
Alcay, Selim ;
Toker, M. Berk ;
Nur, Zekariya ;
Gokce, Elif ;
Sonat, Fusun Ak ;
Gul, Zulfiye ;
Duman, Muhammed ;
Cenize, Cafer ;
Uslu, Aydin ;
Sagirkaya, Hakan ;
Soylu, M. Kemal .
ANIMAL REPRODUCTION SCIENCE, 2016, 164 :97-104
[19]   Effect of autologous platelet-rich plasma on the fertility and quality of cryopreserved buffalo bull semen: a comparative study using OptiXcell® and tris egg yolk extenders [J].
Salama, Maha S. ;
Ashour, Mohey A. ;
Taher, Ehab S. ;
Rashed, Fatema ;
Ibrahim, Ibrahim M. ;
El-Nablaway, Mohammad ;
Ibrahim, Ateya Megahed ;
Mihaela, Ostan ;
Olga, Rada ;
Mohammed, Nourelhuda A. ;
Abdeen, Ahmed ;
Shukry, Mustafa .
BMC VETERINARY RESEARCH, 2024, 20 (01)
[20]   Effect of egg yolk and seminal plasma heparin binding protein interaction on the freezability of buffalo cauda epididymal spermatozoa [J].
Singh, L. P. ;
Harshan, Hiron M. ;
Ansari, M. R. .
ANIMAL REPRODUCTION SCIENCE, 2007, 99 (3-4) :395-400