Steinernema australe n. sp (Panagrolaimomorpha: Steinernematidae), a new entomopathogenic nematode from Isla Magdalena, Chile

被引:22
作者
Edgington, Steve [1 ]
Buddie, Alan G. [1 ]
Tymo, Lukasz [1 ]
Hunt, David J. [1 ]
Nguyen, Khuong B. [2 ]
France, Andres I. [3 ]
Merino, Loreto M. [3 ]
Moore, Dave [1 ]
机构
[1] CABI Europe UK, Egham TW20 9TY, Surrey, England
[2] Univ Florida, Gainesville, FL 32611 USA
[3] INIA, Chillan, Chile
关键词
description; molecular; morphology; new species; phylogeny; SEM; taxonomy; SP RHABDITIDA; PHYLOGENETIC-RELATIONSHIPS; BEETLE LARVAE; METARHIZIUM; COLEOPTERA; TRAVASSOS; SEQUENCES; REGION; RDNA;
D O I
10.1163/156854108X399326
中图分类号
Q95 [动物学];
学科分类号
071002 ;
摘要
A new species of entomopathogenic nematode, Steinernema australe n. sp., was isolated from a soil sample taken close to the beach on Isla Magdalena, an island in the Pacific Ocean, 2 km from mainland Chile. Morphologically the new species belongs to the glaseri-group and is characterised by morphometrics of the infective juvenile which has a very long body of 1316 (1162-1484) mu m, excretory pore located far posterior to the anterior extremity (110 (95-125) mu m), exceptionally long tail of 103 (92-114) mu m, H% = 51 (42-61), E% = 107 (94-122) and a ratio = 35 (31-38). The first generation male has 72 (55-78) mu m long spicules, a 45 (36-51) mu m long gubernaculum and SW% = 172 (118-196). The first generation female can be recognised by well developed double epiptygmata, the lack of a prominent postanal swelling, a mucron on the tail tip and (in 60% of individuals) one to two subsidiary mucrons. Sequences of the ITS and D2D3 regions of the ribosomal DNA confirm that S. australe n. sp. is a valid species.
引用
收藏
页码:699 / 717
页数:19
相关论文
共 48 条
  • [1] Aguera de Doucet M. M., 1986, Revue de Nematologie, V9, P317
  • [2] Akhurst R., 2002, Entomopathogenic nematology, P311, DOI 10.1079/9780851995670.0311
  • [3] [Anonymous], 1989, Molecular Cloning: A Laboratory Manual
  • [4] [Anonymous], 2002, MACCLADE VERSION 4 0
  • [5] Artyukhovsky AK, 1967, T VORONESZHSKOGO GOS, V15, P94
  • [6] SIMPLE TECHNIQUE FOR DETECTION OF INSECT PARASITIC RHABDITID NEMATODES IN SOIL
    BEDDING, RA
    AKHURST, RJ
    [J]. NEMATOLOGICA, 1975, 21 (01): : 109 - 110
  • [7] PHYLOGENY OF METARHIZIUM - ANALYSIS OF RIBOSOMAL DNA-SEQUENCE DATA
    CURRAN, J
    DRIVER, F
    BALLARD, JWO
    MILNER, RJ
    [J]. MYCOLOGICAL RESEARCH, 1994, 98 : 547 - 552
  • [8] Cutler G. Christopher, 2003, Nematologia Mediterranea, V31, P215
  • [9] STEINERNEMA-RITTERI) N-SP (NEMATODA, STEINERNEMATIDAE) WITH A KEY TO THE SPECIES OF THE GENUS
    DEDOUCET, MMA
    DOUCET, ME
    [J]. NEMATOLOGICA, 1990, 36 (03): : 257 - 265
  • [10] DEDOUCET MMA, 1994, FUND APPL NEMATOL, V17, P441