Developments in high-throughput functional epigenomics: CRISPR-single-cell assay for transposase-accessible chromatin using sequencing screens

被引:3
作者
Yan, Rachel E. [1 ]
Greenfield, Jeffrey P. [1 ]
Dahmane, Nadia [1 ]
机构
[1] Weill Cornell Med Coll, Dept Neurol Surg, New York, NY 10021 USA
关键词
ATAC; CRISPR; functional genomics; genetic screens; single cell; PROTEINS;
D O I
10.2217/epi-2022-0093
中图分类号
Q3 [遗传学];
学科分类号
071007 ; 090102 ;
摘要
Tweetable abstract CRISPR-scATAC-seq screens pave the way for high-throughput functional epigenomics by linking perturbations to a broad view of epigenetic state and messages hidden within accessible sequences.
引用
收藏
页码:645 / 649
页数:5
相关论文
共 22 条
  • [1] Buenrostro JD, 2013, NAT METHODS, V10, P1213, DOI [10.1038/nmeth.2688, 10.1038/NMETH.2688]
  • [2] The chromatin accessibility landscape of primary human cancers
    Corces, M. Ryan
    Granja, Jeffrey M.
    Shams, Shadi
    Louie, Bryan H.
    Seoane, Jose A.
    Zhou, Wanding
    Silva, Tiago C.
    Groeneveld, Clarice
    Wong, Christopher K.
    Cho, Seung Woo
    Satpathy, Ansuman T.
    Mumbach, Maxwell R.
    Hoadley, Katherine A.
    Robertson, A. Gordon
    Sheffield, Nathan C.
    Felau, Ina
    Castro, Mauro A. A.
    Berman, Benjamin P.
    Staudt, Louis M.
    Zenklusen, Jean C.
    Laird, Peter W.
    Curtis, Christina
    Greenleaf, William J.
    Chang, Howard Y.
    [J]. SCIENCE, 2018, 362 (6413) : 420 - +
  • [3] Lineage-specific and single-cell chromatin accessibility charts human hematopoiesis and leukemia evolution
    Corces, M. Ryan
    Buenrostro, Jason D.
    Wu, Beijing
    Greenside, Peyton G.
    Chan, Steven M.
    Koenig, Julie L.
    Snyder, Michael P.
    Pritchard, Jonathan K.
    Kundaje, Anshul
    Gkeenleaf, William J.
    Majeti, Ravindra
    Chang, Howard Y.
    [J]. NATURE GENETICS, 2016, 48 (10) : 1193 - 1203
  • [4] Multiplex single-cell profiling of chromatin accessibility by combinatorial cellular indexing
    Cusanovich, Darren A.
    Daza, Riza
    Adey, Andrew
    Pliner, Hannah A.
    Christiansen, Lena
    Gunderson, Kevin L.
    Steemers, Frank J.
    Trapnell, Cole
    Shendure, Jay
    [J]. SCIENCE, 2015, 348 (6237) : 910 - 914
  • [5] Datlinger P, 2017, NAT METHODS, V14, P297, DOI [10.1038/NMETH.4177, 10.1038/nmeth.4177]
  • [6] Perturb-Seq: Dissecting Molecular Circuits with Scalable Single-Cell RNA Profiling of Pooled Genetic Screens
    Dixit, Atray
    Pamas, Oren
    Li, Biyu
    Chen, Jenny
    Fulco, Charles P.
    Jerby-Amon, Livnat
    Marjanovic, Nemanja D.
    Dionne, Danielle
    Burks, Tyler
    Raychowdhury, Raktima
    Adamson, Britt
    Norman, Thomas M.
    Lander, Eric S.
    Weissman, Jonathan S.
    Friedman, Nir
    Regev, Aviv
    [J]. CELL, 2016, 167 (07) : 1853 - +
  • [7] Xenopus research: metamorphosed by genetics and genomics
    Harland, Richard M.
    Grainger, Robert M.
    [J]. TRENDS IN GENETICS, 2011, 27 (12) : 507 - 515
  • [8] Mammalian SWI/SNF chromatin remodeling complexes and cancer: Mechanistic insights gained from human genomics
    Kadoch, Cigall
    Crabtree, Gerald R.
    [J]. SCIENCE ADVANCES, 2015, 1 (05):
  • [9] CRISPR in cancer biology and therapy
    Katti, Alyna
    Diaz, Bianca J.
    Caragine, Christina M.
    Sanjana, Neville E.
    Dow, Lukas E.
    [J]. NATURE REVIEWS CANCER, 2022, 22 (05) : 259 - 279
  • [10] Kiani K., 2022, BIORXIV