Potential protection of 2,3,5,4′-tetrahydroxystilbene-2-O-β-D-glucoside against staurosporine-induced toxicity on cultured rat hippocampus neurons

被引:24
作者
Yang, Xiao-Ping [1 ,2 ]
Liu, Tao-Yan [1 ,2 ]
Qin, Xiao-Yan [1 ,2 ]
Yu, Long-Chuan [3 ,4 ]
机构
[1] Minzu Univ China, Coll Life & Environm Sci, Biotechnol Lab, Beijing 100081, Peoples R China
[2] Minzu Univ China, Coll Life & Environm Sci, State Key Lab Chinese Ethn Minor Tradit Med, Beijing 100081, Peoples R China
[3] Peking Univ, Coll Life Sci, Neurobiol Lab, Beijing 100871, Peoples R China
[4] Peking Univ, Coll Life Sci, State Key Lab Biomembrane & Membrane Biotechnol, Beijing 100871, Peoples R China
关键词
THSG; STS; Hippocampal neurons; p-Akt; Bcl(2); pro-Csp3; BETA-INDUCED TOXICITY; TETRAHYDROXYSTILBENE GLUCOSIDE IMPROVES; POLYGONUM-MULTIFLORUM; ALZHEIMERS-DISEASE; MOUSE MODEL; CELLS; APOPTOSIS; MEMORY; CURCUMIN; GROWTH;
D O I
10.1016/j.neulet.2014.05.045
中图分类号
Q189 [神经科学];
学科分类号
071006 ;
摘要
The present study explored the effect of 2,3,5,4'-tetrahydroxystilbene-2-O-beta-D-glucoside (THSG) on the staurosporine (STS)-induced toxicity in cultured rat hippocampal neurons. The results showed that administration of 200 mu M of THSG significantly protected against 0.3 mu M of STS-induced apoptosis in cultured rat hippocampal neurons tested by methyl thiazolyl tetrazolium (MTT) and terminal deoxynucleotidyl transferase-mediated dUTP nick-end labeling (TUNEL) assays. Furthermore, when the Akt signaling pathway was blocked by LY294002, an inhibitor of Phosphatidyl Inositol 3-kinase (PI3K), the protective effects of THSG against STS-induced neurotoxicity were abrogated. We further examined the involvement of PI3K/Akt signaling pathway in THSG protection against STS-induced cytotoxicity on cultured neurons and found that administration of THSG significantly inhibited the STS-induced decreases in the content of phosphoiylated AKt (p-Akt). Moreover, we found that THSG rescued the down-regulation of B cell lymphoma/lewkmia-2 (Bcl(2)) and pro-caspase-3 (pro-Csp3) caused by STS in the neurons. These results indicate that THSG protect the cultured rat hippocampal neurons against STS-induced cytotoxicity and the PI3K/Akt signaling and mitochondrial apoptotic pathways are involved in the THSG-induced protective effects. (C) 2014 Elsevier Ireland Ltd. All rights reserved.
引用
收藏
页码:79 / 83
页数:5
相关论文
共 28 条
[1]  
Cheng Y., 2013, PLOS ONE, V8
[2]   Galanin Protects Amyloid-β-Induced Neurotoxicity on Primary Cultured Hippocampal Neurons of Rats [J].
Cheng, Yong ;
Yu, Long-Chuan .
JOURNAL OF ALZHEIMERS DISEASE, 2010, 20 (04) :1143-1157
[3]   How do BCL-2 proteins induce mitochondrial outer membrane permeabilization? [J].
Chipuk, Jerry E. ;
Green, Douglas R. .
TRENDS IN CELL BIOLOGY, 2008, 18 (04) :157-164
[4]   Akt signalling in health and disease [J].
Hers, Ingeborg ;
Vincent, Emma E. ;
Tavare, Jeremy M. .
CELLULAR SIGNALLING, 2011, 23 (10) :1515-1527
[5]   Tetrahydroxystilbene glucoside improves learning and (or) memory ability of aged rats and may be connected to the APP pathway [J].
Hou, Ying ;
Yang, Qidong ;
Zhou, Lin ;
Du, Xiaoping ;
Li, Min ;
Yuan, Mei ;
Zhou, Zhiwen ;
Li, Zhenguo .
CANADIAN JOURNAL OF PHYSIOLOGY AND PHARMACOLOGY, 2011, 89 (11) :801-809
[6]   Neuroprotective effects of Polygonum multiflorum on nigrostriatal dopaminergic degeneration induced by paraquat and maneb in mice [J].
Li, X ;
Matsumoto, K ;
Murakami, Y ;
Tezuka, Y ;
Wu, YL ;
Kadota, S .
PHARMACOLOGY BIOCHEMISTRY AND BEHAVIOR, 2005, 82 (02) :345-352
[7]   Tetrahydroxystilbene glucoside attenuates MPP+-induced apoptosis in PC12 cells by inhibiting ROS generation and modulating JNK activation [J].
Li, Xiaobing ;
Li, Yan ;
Chen, Jianzong ;
Sun, Jing ;
Li, Xiaofeng ;
Sun, Xin ;
Kang, Xiaogang .
NEUROSCIENCE LETTERS, 2010, 483 (01) :1-5
[8]   Mitochondrial-targeted active Akt protects SH-SY5Y neuroblastoma cells from staurosporine-induced apoptotic cell death [J].
Mookherjee, Paramita ;
Quintanilla, Rodrigo ;
Roh, Myoung-Sun ;
Zmijewska, Anna A. ;
Jope, Richard S. ;
Johnson, Gail V. W. .
JOURNAL OF CELLULAR BIOCHEMISTRY, 2007, 102 (01) :196-210
[9]   Topical application of Polygonum multiflorum extract induces hair growth of resting hair follicles through upregulating Shh and β-catenin expression in C57BL/6 mice [J].
Park, Hye-Jin ;
Zhang, Nannan ;
Park, Dong Ki .
JOURNAL OF ETHNOPHARMACOLOGY, 2011, 135 (02) :369-375
[10]   Protection by tetrahydroxystilbene glucoside against neurotoxicity induced by MPP+: The involvement of PI3K/Akt pathway activation [J].
Qin, Rong ;
Li, Xiaobing ;
Li, Gang ;
Tao, Lizhen ;
Li, Yan ;
Sun, Jing ;
Kang, Xiaogang ;
Chen, Jianzong .
TOXICOLOGY LETTERS, 2011, 202 (01) :1-7