Serological Differentiation of Streptococcus parauberis Strains Isolated from Cultured Japanese Flounder in Japan

被引：22

作者：

Kanai, Kinya ^{[1
]}

Yamada, Miyuki ^{[1
]}

Meng, Fei ^{[2
]}

Takahashi, Ichirou ^{[3
]}

Nagano, Taizo ^{[4
]}

Kawakami, Hidemasa ^{[5
]}

Yamashita, Azumi ^{[5
]}

Matsuoka, Satoru ^{[6
]}

Fukuda, Yutaka ^{[7
]}

Miyoshi, Yasuyuki ^{[7
]}

Takami, Ikuo ^{[8
]}

Nakano, Heiji ^{[9
]}

Hirae, Tatsumu ^{[10
]}

Shutou, Kimihiro ^{[11
]}

Honma, Toshio ^{[11
]}

机构：

[1] Nagasaki Univ, Fac Fisheries, Nagasaki 8528521, Japan

[2] Nagasaki Univ, Grad Sch Sci & Technol, Nagasaki 8528521, Japan

[3] Shimane Prefectural Govt, Hamada Reg Off Fisheries Affairs, Hamada, Shimane 6970041, Japan

[4] Kagawa Prefectural Fisheries Expt Stn, Kagawa 7610111, Japan

[5] Ehime Prefectural Fish Dis Control Ctr, Uwajima, Ehime 7980087, Japan

[6] Ehime Prefectural Chuyo Fisheries Expt Stn, Iyo, Ehime 7993125, Japan

[7] Oita Prefectural Agr Forestry & Fisheries Res Ctr, Fisheries Res Inst, Oita 8792602, Japan

[8] Nagasaki Prefectural Inst Fisheries, Nagasaki 8512213, Japan

[9] Kumamoto Prefectural Fisheries Res Inst, Kumamoto 8693603, Japan

[10] Kagoshima Prefectural Fisheries Technol & Dev Ctr, Kagoshima 8910315, Japan

[11] Bio Sci Co Ltd, Tokushima 7791292, Japan

来源：

FISH PATHOLOGY | 2009年 / 44卷 / 01期

关键词：

Streptococcus parauberis; Paralichthys olivaceus; streptococcicosis; serotype; capsule; POLYACRYLAMIDE GELS; TURBOT; UBERIS; FISH; SUIS;

D O I：

10.3147/jsfp.44.33

中图分类号：

S9 [水产、渔业];

学科分类号：

0908 ;

摘要：

Streptococcus parauberis, a Gram-positive bacterium, is known as the causative agent of mastitis in cows as well as streptococcicosis in turbot Scophthalmus maximus and Japanese flounder Paralichthys olivaceus. It has recently become one of the most important pathogens of cultured Japanese flounder in western Japan. In this study, we investigated the serological nature of S. parauberis strains isolated from Japanese flounder in Japan. As a result, the strains were distinctly divided into two groups, serotype I represented by strain KRS-02083 and serotype II represented by strain KRS-02109, by an agglutination test with rabbit antisera. Autoclave-extracts of bacterial cells derived from each serotype formed a type-specific precipitation line with each corresponding antiserum in an agar double diffusion test. Acidic polysaccharides were detected in the autoclave-extracts of the bacterial cells. Electron microscopy revealed capsular structure on the bacterial cells pretreated with each corresponding antiserum. This finding suggests that capsular polysaccharide antigens serve to differentiate two serotypes.

引用

页码：33 / 39

页数：7

共 12 条

[11] ANTIGENIC CHARACTERIZATION OF ENTEROCOCCUS STRAINS PATHOGENIC FOR TURBOT AND THEIR RELATIONSHIP WITH OTHER GRAM-POSITIVE BACTERIA
TORANZO, AE
CUTRIN, JM
NUNEZ, S
ROMALDE, JL
BARJA, JL
[J]. DISEASES OF AQUATIC ORGANISMS, 1995, 21 (03) : 187 - 191
[12] MOLECULAR TAXONOMIC STUDIES ON STREPTOCOCCUS-UBERIS TYPE-I AND TYPE-II - DESCRIPTION OF STREPTOCOCCUS-PARAUBERIS SP-NOV
WILLIAMS, AM
COLLINS, MD
[J]. JOURNAL OF APPLIED BACTERIOLOGY, 1990, 68 (05): : 485 - 490

← 1 2 →