Speckle-illuminated fluorescence confocal microscopy, using a digital micro-mirror device

被引：22

作者：

Jiang, Shi-Hong ^{[1
]}

GWalker, John ^{[1
]}

机构：

[1] Univ Nottingham, Dept Elect & Elect Engn, Nottingham NG7 2RD, England

来源：

MEASUREMENT SCIENCE AND TECHNOLOGY | 2009年 / 20卷 / 06期

关键词：

confocal microscopy; speckle pattern; digital micro-mirror device (DMD); MICROMIRROR DEVICE; DMD; SYSTEMS;

D O I：

10.1088/0957-0233/20/6/065501

中图分类号：

T [工业技术];

学科分类号：

08 ;

摘要：

An implementation of a speckle-illuminated fluorescence confocal microscope using a digital micro-mirror device (DMD) is described. The DMD not only projects a sequence of imaged binary speckle patterns onto the specimen at a very high frame rate but also operates as a spatial light modulator to perform real-time optical data processing. Frame averaging is accomplished by CCD charge accumulation during a single exposure. The recorded time-averaged image is a confocal image plus an unwanted non-confocal image which can be removed by recording a separate image. Experimental results with image acquisition within a fraction of a second are shown. Images of a thin biological sample are also shown to demonstrate practical application of the technique.

引用

页数：8

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