Backbone 1H, 15N, 13C and side chain 13Cβ NMR chemical shift assignment of murine interleukin-10

被引：5

作者：

Kuenze, Georg ^{[1
]}

Theisgen, Stephan ^{[1
]}

Huster, Daniel ^{[1
]}

机构：

[1] Univ Leipzig, Inst Med Phys & Biophys, D-04107 Leipzig, Germany

来源：

BIOMOLECULAR NMR ASSIGNMENTS | 2014年 / 8卷 / 02期

关键词：

Cytokine; Autoimmunity; NMR; Protein-carbohydrate-interaction; SECONDARY STRUCTURE; INTERFERON-GAMMA; GROWTH-FACTORS; PROTEIN; CYTOKINES; IL-10; GENE;

D O I：

10.1007/s12104-013-9521-3

中图分类号：

Q6 [生物物理学];

学科分类号：

071011 ;

摘要：

Almost complete assignment of backbone H-1, C-13, N-15 and side chain C-13 beta resonances for the immune-regulatory cytokine IL-10 is reported. The protein was overexpressed in Escherichia coli and was refolded from inclusion bodies. The point mutation C149Y was introduced to suppress incorrect disulfide bond formation and to improve protein refolding.

引用

页码：375 / 378

页数：4

共 25 条

[1]

Angulo J, 2006, METHOD ENZYMOL, V416, P12, DOI 10.1016/S0076-6879(06)16002-4

[2] Interleukin-10 therapy - Review of a new approach [J].

Asadullah, K ;

Sterry, W ;

Volk, HD .

PHARMACOLOGICAL REVIEWS, 2003, 55 (02) :241-269

[3]

Ball C, 2001, EUR CYTOKINE NETW, V12, P187

[4] Viral and murine interleukin-10 are correctly processed and retain their biological activity when produced in tobacco [J].

Bortesi, Luisa ;

Rossato, Marzia ;

Schuster, Flora ;

Raven, Nicole ;

Stadlmann, Johannes ;

Avesani, Linda ;

Falorni, Alberto ;

Bazzoni, Flavia ;

Bock, Ralph ;

Schillberg, Stefan ;

Pezzotti, Mario .

BMC BIOTECHNOLOGY, 2009, 9

[5] Biological implications of glycosaminoglycan interactions with haemopoietic cytokines [J].

Coombe, Deirdre R. .

IMMUNOLOGY AND CELL BIOLOGY, 2008, 86 (07) :598-607

[6]

de Waal MR, 1992, CURR OPIN IMMUNOL, V4, P314

[7] The Structure of Glycosaminoglycans and their Interactions with Proteins [J].

Gandhi, Neha S. ;

Mancera, Ricardo L. .

CHEMICAL BIOLOGY & DRUG DESIGN, 2008, 72 (06) :455-482

[8] H-1, C-13, AND N-15 NMR BACKBONE ASSIGNMENTS AND SECONDARY STRUCTURE OF HUMAN INTERFERON-GAMMA [J].

GRZESIEK, S ;

DOBELI, H ;

GENTZ, R ;

GAROTTA, G ;

LABHARDT, AM ;

BAX, A .

BIOCHEMISTRY, 1992, 31 (35) :8180-8190

[9]

KIM JM, 1992, J IMMUNOL, V148, P3618

[10]

MOORE KW, 1993, ANNU REV IMMUNOL, V11, P165, DOI 10.1146/annurev.iy.11.040193.001121

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