Do Pluripotent Stem Cells Exist in Adult Mice as Very Small Embryonic Stem Cells?

被引：59

作者：

Miyanishi, Masanori ^{[1
]}

Mori, Yasuo ^{[1
]}

Seita, Jun ^{[1
]}

Chen, James Y. ^{[1
]}

Karten, Seth ^{[1
]}

Chan, Charles K. F. ^{[1
]}

Nakauchi, Hiromitsu ^{[2
]}

Weissman, Irving L. ^{[1
]}

机构：

[1] Stanford Univ, Sch Med, Inst Stem Cell Biol & Regenerat Med, Stanford, CA 94305 USA

[2] Univ Tokyo, Inst Med Sci, Div Stem Cell Therapy, Ctr Stem Cell Biol & Regenerat Med, Tokyo 1088639, Japan

来源：

STEM CELL REPORTS | 2013年 / 1卷 / 02期

基金：

美国国家卫生研究院; 日本学术振兴会;

关键词：

BONE-MARROW; CORD BLOOD; PERIPHERAL-BLOOD; IDENTIFICATION; LINEAGE; ORGANS; DIFFERENTIATION; TRANSPLANTATION; PLASTICITY; LIVER;

D O I：

10.1016/j.stemcr.2013.07.001

中图分类号：

Q813 [细胞工程];

学科分类号：

摘要：

Very small embryonic-like stem cells (VSELs) isolated from bone marrow (BM) have been reported to be pluripotent. Given their nonembryonic source, they could replace blastocyst-derived embryonic stem cells in research and medicine. However, their multiple-germ-layer potential has been incompletely studied. Here, we show that we cannot find VSELs in mouse BM with any of the reported stem cell potentials, specifically for hematopoiesis. We found that: (1) most events within the "VSEL'' flow-cytometry gate had little DNA and the cells corresponding to these events (2) could not form spheres, (3) did not express Oct4, and (4) could not differentiate into blood cells. These results provide a failure to confirm the existence of pluripotent

引用

页码：198 / 208

页数：11

共 45 条

[1] Raise standards for preclinical cancer research [J].

Begley, C. Glenn ;

Ellis, Lee M. .

NATURE, 2012, 483 (7391) :531-533

[2] Multipotent cells can be generated in vitro from several adult human organs (heart, liver, and bone marrow) [J].

Beltrami, Antonio R. ;

Cesselli, Daniela ;

Bergamin, Natascha ;

Marcon, Patrizia ;

Rigo, Silvia ;

Puppato, Elisa ;

D'Aurizio, Federica ;

Verardo, Roberto ;

Piazza, Silvano ;

Pignatelli, Angela ;

Poz, Alessandra ;

Baccarani, Umberto ;

Damiani, Daniela ;

Fanin, Renato ;

Mariuzzi, Laura ;

Finato, Nicoletta. ;

Masolini, Paola ;

Burelli, Silvia ;

Belfuzzi, Ottorino ;

Schneider, Claudio ;

Beltrami, Carlo A. .

BLOOD, 2007, 110 (09) :3438-3446

[3] Very Small Embryonic-Like Stem Cells with Maximum Regenerative Potential Get Discarded During Cord Blood Banking and Bone Marrow Processing for Autologous Stem Cell Therapy [J].

Bhartiya, Deepa ;

Shaikh, Ambreen ;

Nagvenkar, Punam ;

Kasiviswanathan, Sandhya ;

Pethe, Prasad ;

Pawani, Harsha ;

Mohanty, Sujata ;

Rao, S. G. Ananda ;

Zaveri, Kusum ;

Hinduja, Indira .

STEM CELLS AND DEVELOPMENT, 2012, 21 (01) :1-6

[4] The hard copy [J].

Check, Erika .

NATURE, 2007, 446 (7135) :485-486

[5] Very Small Embryonic-Like Stem Cells Purified from Umbilical Cord Blood Lack Stem Cell Characteristics [J].

Danova-Alt, Ralitza ;

Heider, Andreas ;

Egger, Dietmar ;

Cross, Michael ;

Alt, Ruediger .

PLOS ONE, 2012, 7 (04)

[6] Systemic overexpression of BCL-2 in the hematopoietic system protects transgenic mice from the consequences of lethal irradiation [J].

Domen, J ;

Gandy, KL ;

Weissman, IL .

BLOOD, 1998, 91 (07) :2272-2282

[7] Stem Cells, Including a Population of Very Small Embryonic-Like Stem Cells, are Mobilized Into Peripheral Blood in Patients After Skin Burn Injury [J].

Drukala, Justyna ;

Paczkowska, Edyta ;

Kucia, Magda ;

Mlynska, Elzbieta ;

Krajewski, Andrzej ;

Machalinski, Boguslaw ;

Madeja, Zbigniew ;

Ratajczak, Mariusz Z. .

STEM CELL REVIEWS AND REPORTS, 2012, 8 (01) :184-194

[8] Concerted action of Smad and CREB-binding protein regulates bone morphogenetic protein-2-stimulated osteoblastic colony-stimulating factor-1 expression [J].

Ghosh-Choudhury, Nandini ;

Singha, Prajjal K. ;

Woodruff, Kathleen ;

St Clair, Patricia ;

Bsoul, Sameer ;

Werner, Sherry L. ;

Choudhury, Goutam Ghosh .

JOURNAL OF BIOLOGICAL CHEMISTRY, 2006, 281 (29) :20160-20170

[9] An improved mouse Sca-1+ cell-based bone marrow transplantation model for use in gene- and cell-based therapeutic studies [J].

Hall, Susan L. ;

Lau, K. -H. William ;

Chen, Shin-Tai ;

Felt, Joshua C. ;

Gridley, Daila S. ;

Yee, Jiing-Kuan ;

Baylink, David J. .

ACTA HAEMATOLOGICA, 2007, 117 (01) :24-33

[10] Interpreting flow cytometry data: a guide for the perplexed [J].

Herzenberg, Leonore A. ;

Tung, James ;

Moore, Wayne A. ;

Herzenberg, Leonard A. ;

Parks, David R. .

NATURE IMMUNOLOGY, 2006, 7 (07) :681-685

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