Direct measurement of the release of atp and its major metabolites from the nerve fibres of the guinea-pig taenia coli

被引:23
作者
McConalogue, K
Todorov, L
Furness, JB
Westfall, DP
机构
[1] UNIV MELBOURNE,DEPT ANAT & CELL BIOL,PARKVILLE,VIC 3052,AUSTRALIA
[2] UNIV NEVADA,DEPT PHARMACOL,RENO,NV 89557
关键词
adenosine triphosphate; enteric nervous system; neurotransmitter release; purinergic nerves; taenia coli;
D O I
10.1111/j.1440-1681.1996.tb01184.x
中图分类号
R9 [药学];
学科分类号
1007 ;
摘要
1. The release of adenosine triphosphate (ATP), adenosine diphosphate, adenosine monophosphate and adenosine from guinea-pig taenia coli in response to electrical stimulation of intramural nerves was measured directly using high performance liquid chromatography separation and fluorometric detection. 2. Purines were released in a frequency-dependent manner by trains of transmural electrical pulses at 1-30 Hz. 3. Electrically evoked release of ATP was abolished by tetrodotoxin (10(-6) mol/L) but was not affected by nicardipine (10(-6) mol/L). 4. The release of purines was reduced in the presence of atropine. 5. Pituitary adenylyl cyclase-activating peptide did not evoke the release of any purines and did not modify the electrically evoked release of purines. 6. The results suggest that ATP and its major metabolites are released from a neuronal source, possibly the enteric inhibitory nerves, in the guinea-pig taenia coli.
引用
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页码:807 / 812
页数:6
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