A benzothiazole-based fluorescent probe for efficient detection and discrimination of Zn2+ and Cd2+, using cysteine as an auxiliary reagent

被引：76

作者：

Li, Jia ^{[1
]}

Chen, Yahui ^{[1
]}

Chen, Tiantian ^{[1
]}

Qiang, Jian ^{[1
]}

Zhang, Zhijie ^{[1
]}

Wei, Tingwen ^{[1
]}

Zhang, Wei ^{[1
]}

Wang, Fang ^{[1
]}

Chen, Xiaoqiang ^{[1
]}

机构：

[1] Nanjing Tech Univ, Jiangsu Natl Synerget Innovat Ctr Adv Mat SICAM, Coll Chem Engn, State Key Lab Mat Oriented Chem Engn, Nanjing 210009, Jiangsu, Peoples R China

来源：

SENSORS AND ACTUATORS B-CHEMICAL | 2018年 / 268卷

基金：

中国国家自然科学基金;

关键词：

Benzothiazole-based derivative; Fluorescent sensor; Zn/Cd ions; Biological imaging; Cysteine; LIVING CELLS; AQUEOUS-SOLUTION; ZINC IONS; DISTINGUISHING CADMIUM; RATIOMETRIC DETECTION; EXCIMER FORMATION; SENSOR; CHEMOSENSORS; RECOGNITION; QUINOLINE;

D O I：

10.1016/j.snb.2018.04.130

中图分类号：

O65 [分析化学];

学科分类号：

070302 ; 081704 ;

摘要：

This paper describes the design and synthesis of a benzothiazole-based fluorescent probe (H-1) for zinc and cadmium ion detection and discrimination, using cysteine as an auxiliary reagent. Among the various metal ions tested, zinc and cadmium ions induced blue-shifted emissions of probe H-1 from 573 nm to 520 nm and 540 nm, respectively. The addition of cysteine caused the emission of H-1-Cd2+ to red-shift back to 573 nm. In contrast, the emission of H-1-Zn2+ with the addition of cysteine only decreased without anyred shift of wavelength. Furthermore, confocal laser scanning micrographs of HeLa cells demonstrated satisfactory cell permeability of H-1 and selectivity toward zinc and cadmium ions in living cells. Test strip experiments suggest that probe H-1 can recognise zinc and cadmium ions together by a remarkable fluorescence change, thus providing a convenient method for tracking zinc and cadmium ions in biological systems. (c) 2018 Elsevier B.V. All rights reserved.

引用

页码：446 / 455

页数：10

共 47 条

[31] Imaging of Zn2+ release from pancreatic β-cells at the level of single exocytotic events [J].