Glycan Analysis of Prostate Specific Antigen (PSA) Directly from the Intact Glycoprotein by HR-ESI/TOF-MS

被引：32

作者：

Behnken, Henning N. ^{[1
]}

Ruthenbeck, Alexandra ^{[1
]}

Schulz, Jan-Mirco ^{[1
]}

Meyer, Bernd ^{[1
]}

机构：

[1] Univ Hamburg, Dept Chem, D-20144 Hamburg, Germany

来源：

JOURNAL OF PROTEOME RESEARCH | 2014年 / 13卷 / 02期

关键词：

glycan; glycoprotein; high resolution mass spectrometry; prostate specific antigen; glycan profiling; MASS-SPECTROMETRY; SEMINAL PLASMA; GLYCOSYLATION;

D O I：

10.1021/pr400999y

中图分类号：

Q5 [生物化学];

学科分类号：

071010 ; 081704 ;

摘要：

Glycans are important modulators of the biological function of proteins and are normally characterized from proteolytic glycopeptidcs or from (N-)glycans released enzymatically by glycosidase treatment or chemically by hydrazinolysis. We demonstrate that glycan compositions can easily be determined directly by LC-ESI/TOF-MS from intact glycoproteins even with a very complex glycosylation pattern. Interpretation of isotopically resolved mass spectra of prostate specific antigen (PSA) using bioinformatics tools gives within a few hours the glycan compositions of 38 glycoforms.

引用

页码：997 / 1001

页数：5

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