Bioaffinity separation of chymotrypsinogen using antigen-antibody reaction in reverse micellar system composed of a nonionic surfactant

被引：15

作者：

Adachi, M ^{[1
]}

Harada, M

Katoh, S

机构：

[1] Kyoto Univ, Inst Adv Energy, Uji, Kyoto 6110011, Japan

[2] Kobe Univ, Dept Sci & Chem Engn, Kobe, Hyogo 6578501, Japan

来源：

BIOCHEMICAL ENGINEERING JOURNAL | 2000年 / 4卷 / 02期

关键词：

antigen-antibody interaction; reverse micellar system; chymotrypsinogen; extraction; nonionic surfactant;

D O I：

10.1016/S1369-703X(99)00043-1

中图分类号：

Q81 [生物工程学（生物技术）]; Q93 [微生物学];

学科分类号：

071005 ; 0836 ; 090102 ; 100705 ;

摘要：

Selective separation of chymotrypsinogen using anti-chymotrypsinogen-antibodies as affinity ligands was realized in reverse micellar system composed of a nonionic surfactant tetra-oxyethylenemonodecylether. Antibodies as affinity ligands were immobilized in reverse micelles by combining cholesteryl groups covalently. Selective separation of proteins using bioaffinity ligands was extended to antigen-antibody reaction system, which enables us to choose any kind of target proteins. (C) 2000 Elsevier Science S.A. All rights reserved.

引用

页码：149 / 151

页数：3

共 10 条

[1]

Adachi M, 1998, BIOTECHNOL BIOENG, V58, P649, DOI 10.1002/(SICI)1097-0290(19980620)58:6<649::AID-BIT11>3.0.CO

[2]

2-2

[3]

Adachi M, 1997, BIOTECHNOL BIOENG, V53, P406, DOI 10.1002/(SICI)1097-0290(19970220)53:4<406::AID-BIT8>3.0.CO

[4]

2-R

[5] SOLUBILIZATION MECHANISM OF CYTOCHROME-C IN SODIUM BIS(2-ETHYLHEXYL) SULFOSUCCINATE WATER OIL MICROEMULSION [J].

ADACHI, M ;

HARADA, M .

JOURNAL OF PHYSICAL CHEMISTRY, 1993, 97 (14) :3631-3640

[6] Bioaffinity separation of concanavalin A in reverse micellar system composed of AOT/butanol or non-ionic surfactant [J].

Adachi, M ;

Harada, M ;

Shioi, A ;

Takahashi, H ;

Katoh, S .

JOURNAL OF CHEMICAL ENGINEERING OF JAPAN, 1996, 29 (06) :982-989

[7] N-LAURYLBIOTINAMIDE AS AFFINITY SURFACTANT [J].

COUGHLIN, RW ;

BACLASKI, JB .

BIOTECHNOLOGY PROGRESS, 1990, 6 (04) :307-309

[8] AFFINITY-BASED REVERSED MICELLAR PROTEIN EXTRACTION .1. PRINCIPLES AND PROTEIN LIGAND SYSTEMS [J].

KELLEY, BD ;

WANG, DIC ;

HATTON, TA .

BIOTECHNOLOGY AND BIOENGINEERING, 1993, 42 (10) :1199-1208

[9] AFFINITY-BASED REVERSE MICELLAR EXTRACTION AND SEPARATION (ARMES) - A FACILE TECHNIQUE FOR THE PURIFICATION OF PEROXIDASE FROM SOYBEAN HULLS [J].

PARADKAR, VM ;

DORDICK, JS .

BIOTECHNOLOGY PROGRESS, 1993, 9 (02) :199-203

[10] BIOAFFINITY SEPARATIONS USING REVERSED MICELLAR EXTRACTION [J].

WOLL, JM ;

HATTON, TA ;

YARMUSH, ML .

BIOTECHNOLOGY PROGRESS, 1989, 5 (02) :57-62

← 1 →