Super-resolution localization microscopy with photoactivatable fluorescent marker proteins

被引：21

作者：

Hedde, Per Niklas ^{[1
,2
]}

Nienhaus, G. Ulrich ^{[1
,3
,4
]}

机构：

[1] Karlsruhe Inst Technol, Inst Appl Phys, D-76128 Karlsruhe, Germany

[2] Univ Calif Irvine, Dept Biomed Engn, Fluorescence Dynam Lab, Irvine, CA 92697 USA

[3] Karlsruhe Inst Technol, Inst Toxicol & Genet, D-76021 Karlsruhe, Germany

[4] Univ Illinois, Dept Phys, Urbana, IL 61801 USA

来源：

PROTOPLASMA | 2014年 / 251卷 / 02期

关键词：

Fluorescent proteins; Photoactivation; Optical highlighter proteins; Super-resolution; Localization microscopy; STRUCTURED-ILLUMINATION MICROSCOPY; OPTICAL RECONSTRUCTION MICROSCOPY; GENETICALLY EXPRESSED PROBES; THICK BIOLOGICAL SAMPLES; TO-RED CONVERSION; MONOMERIC RED; LIVE CELLS; GFP-FAMILY; CONFOCAL MICROSCOPY; DIFFRACTION-LIMIT;

D O I：

10.1007/s00709-013-0566-z

中图分类号：

Q94 [植物学];

学科分类号：

071001 ;

摘要：

Fluorescent proteins (FPs) have become popular imaging tools because of their high specificity, minimal invasive labeling and allowing visualization of proteins and structures inside living organisms. FPs are genetically encoded and expressed in living cells, therefore, labeling involves minimal effort in comparison to approaches involving synthetic dyes. Photoactivatable FPs (paFPs) comprise a subclass of FPs that can change their absorption/emission properties such as brightness and color upon irradiation. This methodology has found a broad range of applications in the life sciences, especially in localization-based super-resolution microscopy of cells, tissues and even entire organisms. In this review, we discuss recent developments and applications of paFPs in super-resolution localization imaging.

引用

页码：349 / 362

页数：14

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[41] Dynamic clustered distribution of hemagglutinin resolved at 40 nm in living cell membranes discriminates between raft theories [J].