Overexpression of Fyn Induces Formation of filopodia and lamellipodia in CHO Cells via Rearrangement of F-Actin

被引：0

作者：

An, Lei ^{[1
]}

Huang, Yingxue ^{[1
]}

Song, Lingzhen ^{[1
]}

Zhang, Wei ^{[1
]}

Zhang, Yamei ^{[1
]}

Chen, Shulin ^{[1
]}

Zhao, Shanting ^{[1
]}

机构：

[1] Northwest A&F Univ, Coll Vet Med, Yangling 712100, Shaanxi, Peoples R China

来源：

PAKISTAN VETERINARY JOURNAL | 2014年 / 34卷 / 02期

基金：

国家高技术研究发展计划(863计划);

关键词：

Actin; Filopodia; Fyn; Lamellipodia; Stress fibers; PHOSPHORYLATION; KINASE; ISOFORM; GROWTH;

D O I：

暂无

中图分类号：

S85 [动物医学（兽医学）];

学科分类号：

0906 ;

摘要：

As a main ancillary molecule involved in the regulation of proliferation, differentiation and migration of cortical neurons, Fyn has also been identified as a signal factor in motility and growth involved with cytoskeleton. However, the molecular mechanism of Fyn on cytoskeleton remains unclear. The results showed that the morphology of the CHO cells transfected with the recombination vector changed remarkably. The numbers of stress fibers may be crunched to transformation for generating and supporting the formation of filopodia and lamellipodia, but the changes of vinculin and tubulin are unremarkable. In addition, high concentrations of Fyn cause formation of lamellipodia as well as filopodia. From this research we can draw a conclusion that overexpression of mouse Fyn induces the F-actin cytoskeleton rearrangement and both vinculin and tubulin are absent in the process of Fyn-mediated F-actin reorganization. (C) 2013 PVJ. All rights reserved

引用

页码：175 / 179

页数：5

共 17 条

[1] RETRACTED: One isoform of Arg/Abl2 tyrosine kinase is nuclear and the other seven cytosolic isoforms differently modulate cell morphology, motility and the cytoskeleton (Retracted Article) [J].

Bianchi, Cristina ;

Torsello, Barbara ;

Di Stefano, Vitalba ;

Zipeto, Maria A. ;

Facchetti, Rita ;

Bombelli, Silvia ;

Perego, Roberto A. .

EXPERIMENTAL CELL RESEARCH, 2013, 319 (13) :2091-2102

[2] Fyn Promotes Phosphorylation of Collapsin Response Mediator Protein 1 at Tyrosine 504, a Novel, Isoform-Specific Regulatory Site [J].

Buel, Gwen R. ;

Rush, John ;

Ballif, Bryan A. .

JOURNAL OF CELLULAR BIOCHEMISTRY, 2010, 111 (01) :20-28

[3] Adhesive F-actin Waves: A Novel Integrin-Mediated Adhesion Complex Coupled to Ventral Actin Polymerization [J].

Case, Lindsay B. ;

Waterman, Clare M. .

PLOS ONE, 2011, 6 (11)

[4] Role of Reelin in the development and maintenance of cortical lamination [J].

Frotscher, Michael ;

Chai, Xuejun ;

Bock, Hans H. ;

Haas, Carola A. ;

Foerster, Eckart ;

Zhao, Shanting .

JOURNAL OF NEURAL TRANSMISSION, 2009, 116 (11) :1451-1455

[5]

Lewin B, 2010, ANTICANCER RES, V30, P2591

[6] Filopodia: molecular architecture and cellular functions [J].

Mattila, Pieta K. ;

Lappalainen, Pekka .

NATURE REVIEWS MOLECULAR CELL BIOLOGY, 2008, 9 (06) :446-454

[7] Fyn knock-down increases Aβ, decreases phospho-tau, and worsens spatial learning in 3 x Tg-AD mice [J].

Minami, S. Sakura ;

Clifford, Thomas G. ;

Hoe, Hyang-Sook ;

Matsuoka, Yasuji ;

Rebeck, G. William .

NEUROBIOLOGY OF AGING, 2012, 33 (04) :825.e15-825.e24

[8] Actin dynamics: Growth from dendritic branches [J].

Nicholson-Dykstra, S ;

Higgs, HN ;

Harris, ES .

CURRENT BIOLOGY, 2005, 15 (09) :R346-R357

[9] microRNA-125a-3p reduces cell proliferation and migration by targeting Fyn [J].

Ninio-Many, Lihi ;

Grossman, Hadas ;

Shomron, Noam ;

Chuderland, Dana ;

Shalgi, Ruth .

JOURNAL OF CELL SCIENCE, 2013, 126 (13) :2867-2876

[10] Tau impacts on growth-factor-stimulated actin remodeling [J].

Sharma, Vandana M. ;

Litersky, Joel M. ;

Bhaskar, Kiran ;

Lee, Gloria .

JOURNAL OF CELL SCIENCE, 2007, 120 (05) :748-757

← 1 2 →