The Effectiveness of Multi-Session FMT Treatment in Active Ulcerative Colitis Patients: A Pilot Study

被引：28

作者：

Mankowska-Wierzbicka, Dorota ^{[1
]}

Stelmach-Mardas, Marta ^{[2
]}

Gabryel, Marcin ^{[1
]}

Tomczak, Hanna ^{[3
,4
]}

Skrzypczak-Zielinska, Marzena ^{[5
]}

Zakerska-Banaszak, Oliwia ^{[5
]}

Sowinska, Anna ^{[6
]}

Mahadea, Dagmara ^{[1
]}

Baturo, Alina ^{[1
]}

Wolko, Lukasz ^{[7
]}

Slomski, Ryszard ^{[5
]}

Dobrowolska, Agnieszka ^{[1
]}

机构：

[1] Poznan Univ Med Sci, Heliodor Swiecicki Hosp, Dept Gastroenterol Dietet & Internal Med, Przybyszewskiego 49, PL-60355 Poznan, Poland

[2] Poznan Univ Med Sci, Dept Biophys, Grunwaldzka 6, PL-60780 Poznan, Poland

[3] Poznan Univ Med Sci, Dept Dermatol & Venereol, Przybyszewskiego 49, PL-60355 Poznan, Poland

[4] Swiecicki Univ Hosp, Cent Microbiol Lab, Przybyszewskiego 49, PL-60355 Poznan, Poland

[5] Polish Acad Sci, Inst Human Genet, Strzeszynska 32, PL-60479 Poznan, Poland

[6] Poznan Univ Med Sci, Dept Comp Sci & Stat, Rokietnicka 7, PL-60806 Poznan, Poland

[7] Univ Life Sci, Dept Biochem & Biotechnol, Dojazd 11, PL-60632 Poznan, Poland

来源：

BIOMEDICINES | 2020年 / 8卷 / 08期

关键词：

fecal microbiota transplantation (FMT); inflammatory bowel disease (IBD); ulcerative colitis (UC); gut microbiota; FECAL MICROBIOTA TRANSPLANTATION; CLOSTRIDIUM-DIFFICILE INFECTION; INTESTINAL MICROBIOTA; CROHNS-DISEASE; RECURRENT; EFFICACY; BACTERIA; REMISSION; DYSBIOSIS; CHILDREN;

D O I：

10.3390/biomedicines8080268

中图分类号：

Q5 [生物化学]; Q7 [分子生物学];

学科分类号：

071010 ; 081704 ;

摘要：

The modification of the microbiome through fecal microbiota transplantation (FMT) is becoming a very promising therapeutic option for inflammatory bowel disease (IBD) patients. Our pilot study aimed to assess the effectiveness of multi-session FMT treatment in active ulcerative colitis (UC) patients. Ten patients with UC were treated with multi-session FMT (200 mL) from healthy donors, via colonoscopy/gastroscopy. Patients were evaluated as follows: at baseline, at week 7, and after 6 months, routine blood tests (including C reactive protein (CRP) and calprotectin) were performed. 16S rRNA gene (V3V4) sequencing was used for metagenomic analysis. The severity of UC was classified based on the Truelove-Witts index. The assessment of microbial diversity showed significant differences between recipients and healthy donors. FMT contributed to long-term, significant clinical and biochemical improvement. Metagenomic analysis revealed an increase in the amount ofLactobacillaceaea,Micrococcaceae,Prevotellaceae, andTM7 phylumsp.oral clone EW055during FMT, whereasStaphylococcaceaeandBacillaceaedeclined significantly. A positive increase in the proportion of the generaBifidobacterium,Lactobacillus,Rothia,Streptococcus, andVeillonellaand a decrease inBacillus,Bacteroides, andStaphylococcuswere observed based on the correlation between calprotectin andBacillusandStaphylococcus;ferritin andLactobacillus,Veillonella,andBifidobacteriumabundance was indicated. A positive change in the abundance ofFirmicuteswas observed during FMT and after 6 months. The application of multi-session FMT led to the restoration of recipients' microbiota and resulted in the remission of patients with active UC.

引用

页数：16

共 75 条

[1]

Alam A, 2016, NAT MICROBIOL, V1, DOI [10.1038/NMICROBIOL.2015.21, 10.1038/nmicrobiol.2015.21]

[2] Comparison of the fecal microbiota profiles between ulcerative colitis and Crohn's disease using terminal restriction fragment length polymorphism analysis [J].