PEG thiazolidine-2-thione, a novel reagent for facile protein modification: Conjugation of bovine hemoglobin

被引：44

作者：

Greenwald, RB ^{[1
]}

Pendri, A ^{[1
]}

Martinez, A ^{[1
]}

Gilbert, C ^{[1
]}

Bradley, P ^{[1
]}

机构：

[1] ENZON INC,DEPT FORMULAT & PRECLIN DEV,PISCATAWAY,NJ 08854

来源：

BIOCONJUGATE CHEMISTRY | 1996年 / 7卷 / 06期

关键词：

D O I：

10.1021/bc960059o

中图分类号：

Q5 [生物化学];

学科分类号：

071010 ; 081704 ;

摘要：

A novel PEG linker that employs a thiazolidine-2-thione group has been synthesized. Kinetic studies done on this compound demonstrate a relatively long half-life compared to those of traditional succinimidyl linkers. This new PEG derivative reacts with proteins under mild conditions and was utilized to conjugate bovine hemoglobin (bHb) to provide a PEG amide-linked protein. The physical characteristics of this conjugate were compared with those of the known PEG carbamate-linked bHb.

引用

页码：638 / 641

页数：4

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