Microbeam methodologies as powerful tools in manganese hyperaccumulation research: present status and future directions

被引：25

作者：

Fernando, Denise R. ^{[1
]}

Marshall, Alan ^{[2
]}

Baker, Alan J. M. ^{[3
]}

Mizuno, Takafumi ^{[4
]}

机构：

[1] La Trobe Univ, Dept Bot, Bundoora, Vic 3086, Australia

[2] La Trobe Univ, Analyt Electron Microscopy Facil, Bundoora, Vic 3086, Australia

[3] Univ Melbourne, Sch Bot, Parkville, Vic 3052, Australia

[4] Mie Univ, Grad Sch Bioresources, Tsu, Mie, Japan

来源：

FRONTIERS IN PLANT SCIENCE | 2013年 / 4卷

基金：

日本学术振兴会;

关键词：

Mn hyperaccumulator; microbeam analysis; Gossia; Maytenus cunninghamii; Alyxia rubricaulis; CELLULAR COMPARTMENTATION; METAL HYPERACCUMULATION; ARABIDOPSIS-THALIANA; NICKEL ACCUMULATION; LEAF TISSUES; PLANT; LEAVES; LOCALIZATION; TRANSPORTER; MN;

D O I：

10.3389/fpls.2013.00319

中图分类号：

Q94 [植物学];

学科分类号：

071001 ;

摘要：

Microbeam studies over the past decade have garnered unique insight into manganese (Mn) homeostasis in plant species that hyperaccumulate this essential mineral micronutrient. Electron- and/or proton-probe methodologies employed to examine tissue elemental distributions have proven highly effective in illuminating excess foliar Mn disposal strategies, some apparently unique to Mn hyperaccumulating plants. When applied to samples prepared with minimal artefacts, these are powerful tools for extracting true 'snapshot' data of living systems. For a range of reasons, Mn hyperaccumulation is particularly suited to in vivo interrogation by this approach. Whilst microbeam investigation of metallophytes is well documented, certain methods originally intended for non-biological samples are now widely applied in biology. This review examines current knowledge about Mn hyperaccumulators with reference to microbeam methodologies, and discusses implications for future research into metal transporters.

引用

页数：9