Proteomic analysis of mouse growth plate cartilage

被引：31
作者：
Belluoccio, Daniele
Wilson, Richard
Thornton, David J.
Wallis, Tristan P.
Gorman, Jeffrey J.
Bateman, John F. [1 ]
机构：
[1] Univ Melbourne, Royal Childrens Hosp, Murdoch Childrens Res Inst, Parkville, Vic 3052, Australia
[2] Univ Melbourne, Royal Childrens Hosp, Dept Paediat, Parkville, Vic 3052, Australia
[3] Univ Manchester, Fac Life Sci, Wellcome Trust Ctr Cell & Matrix Res, Manchester, Lancs, England
[4] Queensland Inst Med Res, Brisbane, Qld 4006, Australia
来源：
PROTEOMICS | 2006年 / 6卷 / 24期
关键词：
cartilage; extracellular matrix proteins; IEF optimization; prefractionation;
D O I：
10.1002/pmic.200600191
中图分类号：
Q5 [生物化学];
学科分类号：
071010 ; 081704 ;
摘要：
Cartilage is a highly specialized load-bearing tissue with a small number of cells and a high proportion of extracellular matrix (ECM). The abundance of heavily sulfated proteoglycans and a poorly soluble collagenous ECM presents a major technical challenge to 2-DE. Here we report proteomic analysis of mouse growth plate cartilage using novel methodology for tissue dissection and sample prefractionation. We have successfully resolved cartilage tissue extracts by 2-DE for the first time and identified cartilage ECM proteins by Western blotting and MS/MS.
引用
收藏
页码：6549 / 6553
页数：5
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