Proteomic analysis of mouse growth plate cartilage

被引：31

作者：

Belluoccio, Daniele

Wilson, Richard

Thornton, David J.

Wallis, Tristan P.

Gorman, Jeffrey J.

Bateman, John F. ^{[1
]}

机构：

[1] Univ Melbourne, Royal Childrens Hosp, Murdoch Childrens Res Inst, Parkville, Vic 3052, Australia

[2] Univ Melbourne, Royal Childrens Hosp, Dept Paediat, Parkville, Vic 3052, Australia

[3] Univ Manchester, Fac Life Sci, Wellcome Trust Ctr Cell & Matrix Res, Manchester, Lancs, England

[4] Queensland Inst Med Res, Brisbane, Qld 4006, Australia

来源：

PROTEOMICS | 2006年 / 6卷 / 24期

关键词：

cartilage; extracellular matrix proteins; IEF optimization; prefractionation;

D O I：

10.1002/pmic.200600191

中图分类号：

Q5 [生物化学];

学科分类号：

071010 ; 081704 ;

摘要：

Cartilage is a highly specialized load-bearing tissue with a small number of cells and a high proportion of extracellular matrix (ECM). The abundance of heavily sulfated proteoglycans and a poorly soluble collagenous ECM presents a major technical challenge to 2-DE. Here we report proteomic analysis of mouse growth plate cartilage using novel methodology for tissue dissection and sample prefractionation. We have successfully resolved cartilage tissue extracts by 2-DE for the first time and identified cartilage ECM proteins by Western blotting and MS/MS.

引用

页码：6549 / 6553

页数：5

共 31 条

[1] LOSS OF PHENOTYPIC TRAITS BY DIFFERENTIATED CELLS .3. REVERSIBLE BEHAVIOR OF CHONDROCYTES IN PRIMARY CULTURES [J].