Structure of the O-polysaccharide of the lipopolysaccharide of Azospirillum irakense KBC1

被引：29
作者：
Fedonenko, YP
Konnova, ON
Zatonsky, GV
Shashkov, AS
Konnova, SA
Zdorovenko, EL
Ignatov, VV
Knirel, YA
机构：
[1] Russian Acad Sci, ND Zelinskii Organ Chem Inst, Moscow 119991, Russia
[2] Russian Acad Sci, Inst Biochem & Physiol Plants & Microorganisms, Saratov 410049, Russia
来源：
CARBOHYDRATE RESEARCH | 2004年 / 339卷 / 10期
基金：
俄罗斯基础研究基金会;
关键词：
lipopolysaccharide; O-antigen; bacterial polysaccharide; asymbiotic diazotroph; Azospirillum irakense;
D O I：
10.1016/j.carres.2004.05.013
中图分类号：
Q5 [生物化学]; Q7 [分子生物学];
学科分类号：
071010 ; 081704 ;
摘要：
The O-polysaccharide was isolated from the lipopolysaccharide of the plant-growth-promoting bacterium Azospirillum irakense KBCl and studied by sugar and methylation analyses, Smith degradation and H-1 and C-13 NMR spectroscopy, including H-1,C-13 HSQC and NOESY experiments for linkage and sequence analysis. The following structure of the branched hexasaccharide repeating unit of the O-polysaccharide with an unusually long side chain was established: alpha-D-Galf-(1-->2)-alpha-L-Rhap-(1-->3)-beta-D-Manp-(1-->3)-alpha-L-Rhap-(1-->3) -->4)-alpha-L-Rhap-(1-->3)-beta-D-Galp-(1--> (C) 2004 Elsevier Ltd. All rights reserved.
引用
收藏
页码：1813 / 1816
页数：4
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