Summary of the ISEV workshop on extracellular vesicles as disease biomarkers, held in Birmingham, UK, during December 2017

被引：56

作者：

Clayton, Aled ^{[1
]}

Buschmann, Dominik ^{[2
,3
]}

Byrd, J. Brian ^{[4
]}

Carter, David R. F. ^{[5
]}

Cheng, Lesley ^{[6
]}

Compton, Carolyn ^{[7
]}

Daaboul, George ^{[8
]}

Devitt, Andrew ^{[9
]}

Manuel Falcon-Perez, Juan ^{[10
]}

Gardiner, Chris ^{[11
]}

Gustafson, Dakota ^{[12
]}

Harrison, Paul ^{[13
]}

Helmbrecht, Clemens ^{[14
]}

Hendrix, An ^{[15
,16
]}

Hill, Andrew ^{[6
]}

Hoffman, Andrew ^{[17
]}

Jones, Jennifer C. ^{[18
]}

Kalluri, Raghu ^{[19
]}

Kang, Ji Yoon ^{[20
]}

Kirchner, Benedikt ^{[21
]}

Lasser, Cecilia ^{[22
]}

Lawson, Charlotte ^{[23
]}

Lenassi, Metka ^{[24
]}

Levin, Carina ^{[25
]}

Llorente, Alicia ^{[26
]}

Martens-Uzunova, Elena S. ^{[27
]}

Moeller, Andreas ^{[28
]}

Musante, Luca ^{[29
]}

Ochiya, Takahiro ^{[30
]}

Pink, Ryan C. ^{[5
]}

Tahara, Hidetoshi ^{[31
]}

Wauben, Marca H. M. ^{[32
]}

Webber, Jason P. ^{[1
]}

Welsh, Joshua A. ^{[18
]}

Witwer, Kenneth W. ^{[33
]}

Yin, Hang ^{[34
]}

Nieuwland, Rienk ^{[35
]}

机构：

[1] Cardiff Univ, Sch Med, Tissue Microenvironm Grp, Cardiff, S Glam, Wales

[2] Tech Univ Munich, Div Anim Physiol & Immunol, Munich, Germany

[3] Ludwig Maximilians Univ Munchen, Univ Hosp, Inst Human Genet, Munich, Germany

[4] Univ Michigan, Dept Internal Med, 5570C MSRB 2, Ann Arbor, MI 48109 USA

[5] Oxford Brookes Univ, Dept Biol & Med Sci, Oxford, England

[6] La Trobe Univ, La Trobe Inst Mol Sci, Dept Biochem & Genet, Melbourne, Vic, Australia

[7] Arizona State Univ, SkySong Ctr Innovat, Scottsdale, AZ USA

[8] NanoView Diagnost, Boston, MA USA

[9] Aston Univ, Sch Life & Hlth Sci, Birmingham, W Midlands, England

[10] IKERBASQUE Res Fdn, Exosomes Lab & Metabol Platform, CIC BioGUNE, CIBEREHD, Derio, Spain

[11] UCL, Res Dept Haematol, Haemostasis Res, London, England

[12] Univ Toronto, Dept Lab Med & Pathobiol, Toronto, ON, Canada

[13] Univ Birmingham, Inst Inflammat & Ageing, Coll Med & Dent Sci, Birmingham, W Midlands, England

[14] Particle Metrix GmbH, Meerbusch, Germany

[15] Univ Ghent, Dept Radiat Oncol & Expt Canc Res, Lab Expt Canc Res, Ghent, Belgium

[16] Canc Res Inst Ghent, Ghent, Belgium

[17] Tufts Univ, Cummings Sch Vet Med, Regenerat Med Lab, North Grafton, MA USA

[18] NCI, Dept Vaccine Branch, Bethesda, MD 20892 USA

[19] UT MD Anderson Canc Ctr, Metastasis Res Ctr, Dept Canc Biol, Houston, TX USA

[20] Korea Inst Sci & Technol, Ctr Biomicrosyst, Seoul, South Korea

[21] Tech Univ Munich, TUM Sch Life Sci Weihenstephan, Div Anim Physiol & Immunol, Munich, Germany

[22] Univ Gothenburg, Inst Med, Sahlgrenska Acad, Krefting Res Ctr, Gothenburg, Sweden

[23] Royal Vet Coll, Dept Comparat Biomed Sci, Royal Coll St, London, England

[24] Univ Ljubljana, Inst Biochem, Ljubljana, Slovenia

[25] Technion, Emek Med Ctr, Afula & Bruce Rappaport Fac Med, Haifa, Israel

[26] Oslo Univ Hosp, Dept Mol Cell Biol, Oslo, Norway

[27] Erasmus MC, Dept Urol, Rotterdam, CA, Netherlands

[28] QIMR Berghofer Med Res Inst, Tumour Microenvironm Lab, Herston, Qld, Australia

[29] Univ Virginia, Dept Med, Div Nephrol, Charlottesville, VA USA

[30] Natl Canc Ctr, Div Mol & Cellular Med, Res Inst, Tokyo, Japan

[31] Hiroshima Univ, Dept Cellular & Mol Biol, Inst & Grad Sch Biomed & Hlth Sci, Hiroshima, Japan

[32] Univ Utrecht, Dept Biochem & Cell Biol, Fac Vet Med, Utrecht, Netherlands

[33] Johns Hopkins Univ, Dept Mol & Comparat Pathobiol, Johns Hopkins Inst NanoBio Technol, Baltimore, MD USA

[34] Tsinghua Univ, Sch Pharmaceut Sci, Beijing, Peoples R China

[35] Univ Amsterdam, Acad Med Ctr, Dept Lab Expt Clin Chem, Amsterdam, DE, Netherlands

来源：

JOURNAL OF EXTRACELLULAR VESICLES | 2018年 / 7卷 / 01期

基金：

英国生物技术与生命科学研究理事会;

关键词：

Biomarkers; extracellular vesicles; exosomes; cancer; serum; plasma; urine; CIRCULATING MICROPARTICLES; CANCER; CHALLENGES; METABOLOME;

D O I：

10.1080/20013078.2018.1473707

中图分类号：

Q2 [细胞生物学];

学科分类号：

071009 ; 090102 ;

摘要：

This report summarises the presentations and activities of the ISEV Workshop on extracellular vesicle biomarkers held in Birmingham, UK during December 2017. Among the key messages was broad agreement about the importance of biospecimen science. Much greater attention needs to be paid towards the provenance of collected samples. The workshop also highlighted clear gaps in our knowledge about pre-analytical factors that alter extracellular vesicles (EVs). The future utility of certified standards for credentialing of instruments and software, to analyse EV and for tracking the influence of isolation steps on the structure and content of EVs were also discussed. Several example studies were presented, demonstrating the potential utility for EVs in disease diagnosis, prognosis, longitudinal serial testing and stratification of patients. The conclusion of the workshop was that more effort focused on pre-analytical issues and benchmarking of isolation methods is needed to strengthen collaborations and advance more effective biomarkers.

引用

页数：11

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