Summary of the ISEV workshop on extracellular vesicles as disease biomarkers, held in Birmingham, UK, during December 2017

被引：55

作者：

Clayton, Aled ^{[1
]}

Buschmann, Dominik ^{[2
,3
]}

Byrd, J. Brian ^{[4
]}

Carter, David R. F. ^{[5
]}

Cheng, Lesley ^{[6
]}

Compton, Carolyn ^{[7
]}

Daaboul, George ^{[8
]}

Devitt, Andrew ^{[9
]}

Manuel Falcon-Perez, Juan ^{[10
]}

Gardiner, Chris ^{[11
]}

Gustafson, Dakota ^{[12
]}

Harrison, Paul ^{[13
]}

Helmbrecht, Clemens ^{[14
]}

Hendrix, An ^{[15
,16
]}

Hill, Andrew ^{[6
]}

Hoffman, Andrew ^{[17
]}

Jones, Jennifer C. ^{[18
]}

Kalluri, Raghu ^{[19
]}

Kang, Ji Yoon ^{[20
]}

Kirchner, Benedikt ^{[21
]}

Lasser, Cecilia ^{[22
]}

Lawson, Charlotte ^{[23
]}

Lenassi, Metka ^{[24
]}

Levin, Carina ^{[25
]}

Llorente, Alicia ^{[26
]}

Martens-Uzunova, Elena S. ^{[27
]}

Moeller, Andreas ^{[28
]}

Musante, Luca ^{[29
]}

Ochiya, Takahiro ^{[30
]}

Pink, Ryan C. ^{[5
]}

Tahara, Hidetoshi ^{[31
]}

Wauben, Marca H. M. ^{[32
]}

Webber, Jason P. ^{[1
]}

Welsh, Joshua A. ^{[18
]}

Witwer, Kenneth W. ^{[33
]}

Yin, Hang ^{[34
]}

Nieuwland, Rienk ^{[35
]}

机构：

[1] Cardiff Univ, Sch Med, Tissue Microenvironm Grp, Cardiff, S Glam, Wales

[2] Tech Univ Munich, Div Anim Physiol & Immunol, Munich, Germany

[3] Ludwig Maximilians Univ Munchen, Univ Hosp, Inst Human Genet, Munich, Germany

[4] Univ Michigan, Dept Internal Med, 5570C MSRB 2, Ann Arbor, MI 48109 USA

[5] Oxford Brookes Univ, Dept Biol & Med Sci, Oxford, England

[6] La Trobe Univ, La Trobe Inst Mol Sci, Dept Biochem & Genet, Melbourne, Vic, Australia

[7] Arizona State Univ, SkySong Ctr Innovat, Scottsdale, AZ USA

[8] NanoView Diagnost, Boston, MA USA

[9] Aston Univ, Sch Life & Hlth Sci, Birmingham, W Midlands, England

[10] IKERBASQUE Res Fdn, Exosomes Lab & Metabol Platform, CIC BioGUNE, CIBEREHD, Derio, Spain

[11] UCL, Res Dept Haematol, Haemostasis Res, London, England

[12] Univ Toronto, Dept Lab Med & Pathobiol, Toronto, ON, Canada

[13] Univ Birmingham, Inst Inflammat & Ageing, Coll Med & Dent Sci, Birmingham, W Midlands, England

[14] Particle Metrix GmbH, Meerbusch, Germany

[15] Univ Ghent, Dept Radiat Oncol & Expt Canc Res, Lab Expt Canc Res, Ghent, Belgium

[16] Canc Res Inst Ghent, Ghent, Belgium

[17] Tufts Univ, Cummings Sch Vet Med, Regenerat Med Lab, North Grafton, MA USA

[18] NCI, Dept Vaccine Branch, Bethesda, MD 20892 USA

[19] UT MD Anderson Canc Ctr, Metastasis Res Ctr, Dept Canc Biol, Houston, TX USA

[20] Korea Inst Sci & Technol, Ctr Biomicrosyst, Seoul, South Korea

[21] Tech Univ Munich, TUM Sch Life Sci Weihenstephan, Div Anim Physiol & Immunol, Munich, Germany

[22] Univ Gothenburg, Inst Med, Sahlgrenska Acad, Krefting Res Ctr, Gothenburg, Sweden

[23] Royal Vet Coll, Dept Comparat Biomed Sci, Royal Coll St, London, England

[24] Univ Ljubljana, Inst Biochem, Ljubljana, Slovenia

[25] Technion, Emek Med Ctr, Afula & Bruce Rappaport Fac Med, Haifa, Israel

[26] Oslo Univ Hosp, Dept Mol Cell Biol, Oslo, Norway

[27] Erasmus MC, Dept Urol, Rotterdam, CA, Netherlands

[28] QIMR Berghofer Med Res Inst, Tumour Microenvironm Lab, Herston, Qld, Australia

[29] Univ Virginia, Dept Med, Div Nephrol, Charlottesville, VA USA

[30] Natl Canc Ctr, Div Mol & Cellular Med, Res Inst, Tokyo, Japan

[31] Hiroshima Univ, Dept Cellular & Mol Biol, Inst & Grad Sch Biomed & Hlth Sci, Hiroshima, Japan

[32] Univ Utrecht, Dept Biochem & Cell Biol, Fac Vet Med, Utrecht, Netherlands

[33] Johns Hopkins Univ, Dept Mol & Comparat Pathobiol, Johns Hopkins Inst NanoBio Technol, Baltimore, MD USA

[34] Tsinghua Univ, Sch Pharmaceut Sci, Beijing, Peoples R China

[35] Univ Amsterdam, Acad Med Ctr, Dept Lab Expt Clin Chem, Amsterdam, DE, Netherlands

来源：

JOURNAL OF EXTRACELLULAR VESICLES | 2018年 / 7卷 / 01期

基金：

英国生物技术与生命科学研究理事会;

关键词：

Biomarkers; extracellular vesicles; exosomes; cancer; serum; plasma; urine; CIRCULATING MICROPARTICLES; CANCER; CHALLENGES; METABOLOME;

D O I：

10.1080/20013078.2018.1473707

中图分类号：

Q2 [细胞生物学];

学科分类号：

071009 ; 090102 ;

摘要：

This report summarises the presentations and activities of the ISEV Workshop on extracellular vesicle biomarkers held in Birmingham, UK during December 2017. Among the key messages was broad agreement about the importance of biospecimen science. Much greater attention needs to be paid towards the provenance of collected samples. The workshop also highlighted clear gaps in our knowledge about pre-analytical factors that alter extracellular vesicles (EVs). The future utility of certified standards for credentialing of instruments and software, to analyse EV and for tracking the influence of isolation steps on the structure and content of EVs were also discussed. Several example studies were presented, demonstrating the potential utility for EVs in disease diagnosis, prognosis, longitudinal serial testing and stratification of patients. The conclusion of the workshop was that more effort focused on pre-analytical issues and benchmarking of isolation methods is needed to strengthen collaborations and advance more effective biomarkers.

引用

页数：11

共 31 条

[1] The impact of various preanalytical treatments on the phenotype of small extracellular vesicles in blood analyzed by protein microarray
Baek, Rikke
Sondergaard, Evo K. L.
Varming, Kim
Jorgensen, Malene M.
[J]. JOURNAL OF IMMUNOLOGICAL METHODS, 2016, 438 : 11 - 20
[2] Raise standards for preclinical cancer research
Begley, C. Glenn
Ellis, Lee M.
[J]. NATURE, 2012, 483 (7391) : 531 - 533
[3] Cell-derived vesicles exposing coagulant tissue factor in saliva
Berckmans, Rene J.
Sturk, Auguste
van Tienen, Laurens M.
Schaap, Marianne C. L.
Nieuwland, Rienk
[J]. BLOOD, 2011, 117 (11) : 3172 - 3180
[4] Exosomes provide a protective and enriched source of miRNA for biomarker profiling compared to intracellular and cell-free blood
Cheng, Lesley
Sharples, Robyn A.
Scicluna, Benjamin J.
Hill, Andrew F.
[J]. JOURNAL OF EXTRACELLULAR VESICLES, 2014, 3 (01):
[5] Characterization and deep sequencing analysis of exosomal and non-exosomal miRNA in human urine
Cheng, Lesley
Sun, Xin
Scicluna, Benjamin J.
Coleman, Bradley M.
Hill, Andrew F.
[J]. KIDNEY INTERNATIONAL, 2014, 86 (02) : 433 - 444
[6] Methodological Guidelines to Study Extracellular Vesicles
Coumans, Frank A. W.
Brisson, Alain R.
Buzas, Edit I.
Dignat-George, Francoise
Drees, Esther E. E.
El-Andaloussi, Samir
Emanueli, Costanza
Gasecka, Aleksandra
Hendrix, An
Hill, Andrew F.
Lacroix, Romaric
Lee, Yi
van Leeuwen, Ton G.
Mackman, Nigel
Maeger, Imre
Nolan, John P.
van der Pol, Edwin
Pegtel, D. Michiel
Sahoo, Susmita
Siljander, Pia R. M.
Sturk, Guus
de Wever, Olivier
Nieuwland, Rienk
[J]. CIRCULATION RESEARCH, 2017, 120 (10) : 1632 - 1648
[7] The end of AIDS: HIV infection as a chronic disease
Deeks, Steven G.
Lewin, Sharon R.
Havlir, Diane V.
[J]. LANCET, 2013, 382 (9903) : 1525 - 1533
[8] Viral protein Nef is detected in plasma of half of HIV-infected adults with undetectable plasma HIV RNA
Ferdin, Jana
Goricar, Katja
Dolzan, Vita
Plemenitas, Ana
Martin, Jeffrey N.
Peterlin, Boris M.
Deeks, Steven G.
Lenassi, Metka
[J]. PLOS ONE, 2018, 13 (01):
[9] New evidence that a large proportion of human blood plasma cell-free DNA is localized in exosomes
Fernando, M. Rohan
Jiang, Chao
Krzyzanowski, Gary D.
Ryan, Wayne L.
[J]. PLOS ONE, 2017, 12 (08):
[10] A signal-amplifiable biochip quantifies extracellular vesicle-associated RNAs for early cancer detection
Hu, Jiaming
Sheng, Yan
Kwak, Kwang Joo
Shi, Junfeng
Yu, Bohao
Lee, L. James
[J]. NATURE COMMUNICATIONS, 2017, 8

← 1 2 3 4 →