Immunohistochemical localization of dopamine D2 receptor in the rat carotid body

被引：11

作者：

Wakai, Jun ^{[1
]}

Takayama, Anna ^{[2
]}

Yokoyama, Takuya ^{[2
]}

Nakamuta, Nobuaki ^{[2
]}

Kusakabe, Tatsumi ^{[3
]}

Yamamoto, Yoshio ^{[2
]}

机构：

[1] Fukushima Med Univ, Sch Med, Lab Anim Res Ctr, Fukushima, Japan

[2] Iwate Univ, Lab Vet Anat & Cell Biol, Fac Agr, Morioka, Iwate 0208550, Japan

[3] Kokushikan Univ, Dept Sport & Med Sci, Lab Anat & Physiol, Tama, Japan

来源：

ACTA HISTOCHEMICA | 2015年 / 117卷 / 08期

基金：

日本学术振兴会;

关键词：

Dopamine D2 receptor; Carotid body; Glomus cell; Chemoreceptor; Rat; MESSENGER-RNA; CATECHOLAMINE SYNTHESIS; TYROSINE-HYDROXYLASE; GLOMUS CELLS; HYPOXIA; EXPRESSION; RELEASE; NEURONS; ACETYLCHOLINE; ADULT;

D O I：

10.1016/j.acthis.2015.07.007

中图分类号：

Q2 [细胞生物学];

学科分类号：

071009 ; 090102 ;

摘要：

Dopamine modulates the chemosensitivity of arterial chemoreteptors, and dopamine D2 receptor (D2R) is expected to localize in the glomus cells and/or sensory nerve endings of the carotid body. In the present study, the localization of D2R in the rat carotid body was examined using double immunofluorescence for D2R with various cell markers. D2R immunoreactivity was mainly localized in glomus cells immunoreactive to tyrosine hydroxylase or dopamine beta-hydroxylase (DBH), but not in S100B-immunoreactive sustentacular cells. Furthermore, D2R immunoreactivity was observed in petrosal ganglion cells and nerve bundles in the carotid body, but not in the nerve endings with P2X2 immunoreactivity. In the carotid ganglion, a few punctate D2R-immunoreactive products were detected in DBH-immunoreactive nerve cell bodies. These results showed that D2R was mainly distributed in glomus cells, and suggested that D2R plays a role in the inhibitory modulation of chemosensory activity in a paracrine and/or autocrine manner. (C) 2015 Elsevier GmbH. All rights reserved.

引用

页码：784 / 789

页数：6

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