Rapid phenotyping of cancer stem cells using multichannel nanosensor arrays

被引：22

作者：

Geng, Yingying ^{[1
]}

Goel, Hira L. ^{[2
]}

Le, Ngoc B. ^{[3
]}

Yoshii, Tatsuyuki ^{[3
,4
]}

Mout, Rubul ^{[3
]}

Tonga, Gulen Y. ^{[3
,5
]}

Amante, John J. ^{[2
]}

Mercurio, Arthur M. ^{[2
]}

Rotello, Vincent M. ^{[3
]}

机构：

[1] Univ Massachusetts, Mol & Cellular Biol Program, Amherst, MA 01003 USA

[2] Univ Massachusetts, Sch Med, Dept Mol Cell & Canc Biol, Worcester, MA USA

[3] Univ Massachusetts, Dept Chem, Amherst, MA 01003 USA

[4] Nagoya Inst Technol, Dept Life Sci Appl Chem, Nagoya, Aichi, Japan

[5] Boston Childrens Hosp, Dept Anaesthesia, Boston, MA USA

来源：

NANOMEDICINE-NANOTECHNOLOGY BIOLOGY AND MEDICINE | 2018年 / 14卷 / 06期

基金：

美国国家卫生研究院;

关键词：

Cancer stem cell; Array-based sensing; Discrimination; Patient-derived xenografts; Personalized medicine; EPITHELIAL-MESENCHYMAL TRANSITION; GENE-EXPRESSION SIGNATURE; BREAST-CANCER; DRUG-RESISTANCE; METASTATIC CELLS; AC133; EPITOPE; CD133; PROTEIN; DIFFERENTIATION; PATHWAY; NANOMATERIALS;

D O I：

10.1016/j.nano.2018.05.009

中图分类号：

TB3 [工程材料学];

学科分类号：

0805 ; 080502 ;

摘要：

Cancer stem cells (CSCs) contribute to multidrug resistance, tumor recurrence and metastasis, making them prime therapeutic targets. Their ability to differentiate and lose stem cell properties makes them challenging to study. Currently, there is no simple assay that can quickly capture and trace the dynamic phenotypic changes on the CSC surface. Here, we report rapid discrimination of breast CSCs from non-CSCs using a nanoparticle-fluorescent-protein based sensor. This nanosensor was employed to discriminate CSCs from non-CSCs, as well as CSCs that had differentiated in vitro in two breast cancer models. Importantly, the sensor platform could also discriminate CSCs from the bulk population of cells in patient-derived xenografts of human breast cancer. Taken together, the results obtained demonstrate the feasibility of using the nanosensor to phenotype CSCs and monitor their fate. Furthermore, this approach provides a novel area for therapeutic interventions against these challenging targets. (C) 2018 Elsevier Inc. All rights reserved.

引用

页码：1931 / 1939

页数：9

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