Anthelmintic activity of the methanol extracts of selected Czech plants using Egg hatch test

The methanol extracts of 11 Czech plants with potential effects against diseases of the gastrointestinal tract were examined to evaluate the anthelmintic activity against Haemonchus contortus. The following plants were selected: Achillea millefolium, Allium sativum, Artemisia absinthium, Cichorium intybus, Matricaria chamomilla, Onobrychis viciifolia, Plantago lanceolata, Quercus robur, Rumex obtusifolius, Urtica dioica and Vicia faba. The extracts were tested at concentrations of 1; 4; 16; 64; 256 a 1024 mu g/ml. In vitro study - Egg hatch test (EHT) was used for evaluation of ovicidal activity. The most effective ovicidal activity against H. contortus were observe in the extract of Allium sativum at concentration of 1024 mu g/ml, 82,7 % of eggs faild to hatch. The value of EC50 was for this plant 796,8 mu g /ml. Other extracts have shown no significant anthelmintic activity, active substances in this extracts supressed larvae hatching from eggs in the range from 0,7 to 51,2 %. Values of EC50 in the individual extracts reached from 1032 mu g /ml to 1115902 mu g /ml.