High-level Production of Recombinant Membrane Proteins Using the Engineered Escherichia coli Strains SuptoxD and SuptoxR

被引：1
作者：
Michou, Myrsini [1 ,2 ]
Delivoria, Dafni C. [1 ]
Skretas, Georgios [1 ]
机构：
[1] Natl Hellen Res Fdn, Inst Chem Biol, Athens 11635, Greece
[2] Univ Thessaly, Dept Biochem & Biotechnol, Viopolis 41500, Larisa, Greece
来源：
BIO-PROTOCOL | 2020年 / 10卷 / 15期
基金：
欧洲研究理事会;
关键词：
Recombinant protein production; Membrane protein; Toxicity; Escherichia coli; SuptoxD; SuptoxR; OVEREXPRESSION; RRAA;
D O I：
10.21769/BioProtoc.3710
中图分类号：
Q [生物科学];
学科分类号：
07 ; 0710 ; 09 ;
摘要：
We have previously described the development of two specialized Escherichia coli strains for high-level recombinant membrane protein (MP) production. These engineered strains, termed SuptoxD and SuptoxR, are capable of suppressing the cytotoxicity caused by MP overexpression and of producing greatly enhanced MP yields. Here, we present a Bio-protocol that describes gene overexpression and culturing conditions that maximize the accumulation of membrane-integrated and well-folded recombinant MPs in these strains.
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页数：14
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