A Microfluidic Chip with Double-Slit Arrays for Enhanced Capture of Single Cells

被引：9

作者：

Xu, Jingyi ^{[1
]}

Chen, Shulei ^{[1
]}

Wang, Dongyang ^{[1
]}

Jiang, Yue ^{[1
]}

Hao, Ming ^{[1
]}

Du, Guangyu ^{[1
]}

Ba, Dechun ^{[1
]}

Lin, Qiao ^{[2
]}

Mei, Qi ^{[3
]}

Ning, Yingchao ^{[4
]}

Su, Da ^{[5
]}

Liu, Kun ^{[1
]}

机构：

[1] Northeastern Univ, Sch Mech Engn & Automat, Shenyang 110819, Liaoning, Peoples R China

[2] Columbia Univ, Dept Mech Engn, New York, NY 10027 USA

[3] Huazhong Univ Sci & Technol, Tongji Hosp, Tongji Med Coll, Dept Oncol, Wuhan 430030, Hubei, Peoples R China

[4] Aviat Ind Corp China, Shenyang Aeroengine Res Inst, Shenyang 110015, Liaoning, Peoples R China

[5] China Natl Heavy Duty Truck Grp Co Ltd, Jinan 250101, Shandong, Peoples R China

来源：

MICROMACHINES | 2018年 / 9卷 / 04期

关键词：

microfluidic chip; hydrodynamic cell trapping; single cell; cell capture; drug delivery; DEFORMATION; FUTURE;

D O I：

10.3390/mi9040157

中图分类号：

O65 [分析化学];

学科分类号：

070302 ; 081704 ;

摘要：

The application of microfluidic technology to manipulate cells or biological particles is becoming one of the rapidly growing areas, and various microarray trapping devices have recently been designed for high throughput single-cell analysis and manipulation. In this paper, we design a double-slit microfluidic chip for hydrodynamic cell trapping at the single-cell level, which maintains a high capture ability. The geometric effects on flow behaviour are investigated in detail for optimizing chip architecture, including the flow velocity, the fluid pressure, and the equivalent stress of cells. Based on the geometrical parameters optimized, the double-slit chip enhances the capture of HeLa cells and the drug experiment verifies the feasibility of the drug delivery.

引用

页数：13

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