Integrated fiducial sample mount and software for correlated microscopy

被引:1
|
作者
McJunkin, Timothy R. [1 ]
Trowbridge, Tammy L. [1 ]
Wright, Karen E. [1 ]
Scott, Jill R. [1 ]
机构
[1] Idaho Natl Lab, Idaho Falls, ID 83415 USA
基金
美国能源部;
关键词
TRANSFORM MASS-SPECTROMETER; 3-DIMENSIONAL REGISTRATION; ELECTRON-MICROSCOPE; MARKERS; SURFACE; IMAGES; LIGHT;
D O I
10.1063/1.4862935
中图分类号
TH7 [仪器、仪表];
学科分类号
0804 ; 080401 ; 081102 ;
摘要
A novel sample mount design with integrated fiducial marks and software for assisting operators in easily and efficiently locating points of interest established in previous analytical sessions is described. The sample holder and software were evaluated with experiments to demonstrate the utility and ease of finding the same points of interest in two different microscopy instruments. Also, numerical analysis of expected errors in determining the same position with errors unbiased by a human operator was performed. Based on the results, issues related to acquiring reproducibility and best practices for using the sample mount and software were identified. Overall, the sample mount methodology allows data to be efficiently and easily collected on different instruments for the same sample location. (C) 2014 AIP Publishing LLC.
引用
收藏
页数:10
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