TCA Precipitation

被引：129

作者：

Koontz, Laura ^{[1
]}

机构：

[1] Johns Hopkins Sch Med, Dept Mol Biol & Genet, Baltimore, MD 21205 USA

来源：

LABORATORY METHODS IN ENZYMOLOGY: PROTEIN, PT C | 2014年 / 541卷

关键词：

D O I：

10.1016/B978-0-12-420119-4.00001-X

中图分类号：

Q5 [生物化学];

学科分类号：

071010 ; 081704 ;

摘要：

Trichloroacetic acid (TCA) precipitation of proteins is commonly used to concentrate protein samples or remove contaminants, including salts and detergents, prior to downstream applications such as SDS-PAGE or 2D-gels. TCA precipitation denatures the protein, so it should not be used if the protein must remain in its folded state (e.g., if you want to measure a biochemical activity of the protein).

引用

页码：3 / 10

页数：8