Antibiotic Resistance Characterization of Environmental E-coli Isolated from River Mula-Mutha, Pune District, India

被引:29
作者
Dhawde, Rutuja [1 ]
Macaden, Ragini [2 ]
Saranath, Dhananjaya [3 ]
Nilgiriwala, Kayzad [1 ]
Ghadge, Appasaheb [4 ]
Birdi, Tannaz [1 ]
机构
[1] Fdn Med Res, 84A RG Thadani Marg, Bombay 400018, Maharashtra, India
[2] St Johns Res Inst, 100 Feet Rd, Bangalore 560034, Karnataka, India
[3] CPAA, Bombay 4000018, Maharashtra, India
[4] Fdn Res Community Hlth, Pune 411007, Maharashtra, India
关键词
Mula-Mutha river; antibiotic susceptibility testing; antibiotic-resistant genes; integrons; QUINOLONE RESISTANCE; WASTE-WATER; TREATMENT PLANTS; BETA-LACTAMASES; SHIGA TOXIN; PREVALENCE; INTEGRONS; GENES; PLASMID; DETERMINANTS;
D O I
10.3390/ijerph15061247
中图分类号
X [环境科学、安全科学];
学科分类号
08 ; 0830 ;
摘要
In the current study, ceftazidime- and ciprofloxacin-resistantor dual drug-resistant (DDR)E. coli were isolated from river Mula-Mutha, which flows through rural Pune district and Pune city. The DDR E. coli were further examined for antibiotic resistance to six additional antibiotics. The study also included detection of genes responsible for ceftazidime and ciprofloxacin resistance and vectors for horizontal gene transfer. Twenty-eight percent of the identified DDR E. coli were resistant to more than six antibiotics, with 12% being resistant to all eight antibiotics tested. Quinolone resistance was determined through the detection of qnrA, qnrB, qnrS and oqxA genes, whereas cephalosporin resistance was confirmed through detection of TEM, CTX-M-15, CTX-M-27 and SHV genes. Out of 219 DDR E. coli, 8.2% were qnrS positive and 0.4% were qnrB positive. Percentage of isolates positive for the TEM, CTX-M-15 and CTX-M-27 genes were 32%, 46% and 0.9%, respectively. None of the DDR E. coli tested carried the qnrA, SHV and oqxA genes. Percentage of DDR E. coli carrying Class 1 and 2 integrons (mobile genetic elements) were 47% and 8%, respectively. The results showed that antibiotic resistance genes (ARGs) and integrons were present in the E. coli isolated from the river at points adjoining and downstream of Pune city.
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页数:15
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