Magnetic polysiloxane-polyvinyl alcohol composite as solid-phase in chemiluminescent assays

被引：8
作者：
Coêlho, RAL
Jaques, GA
Barbosa, AD
Velazquez, G
Montenegro, SML
Azevedo, WM
Carvalho, LB
机构：
[1] Univ Fed Pernambuco, Lab Imunopatol Keizo Asami, BR-50670420 Recife, PE, Brazil
[2] Univ Fed Pernambuco, Dept Bioquim, BR-50670420 Recife, PE, Brazil
[3] Univ Nacl Asuncion, Inst Invest Ciencias Salud, Asuncion, Paraguay
[4] Fiocruz MS, Ctr Pesquisas Aggeu Magalhaes, Pernambuco, Brazil
[5] Univ Fed Pernambuco, Dept Quim Fundamental, BR-50670420 Recife, PE, Brazil
来源：
BIOTECHNOLOGY LETTERS | 2002年 / 24卷 / 20期
关键词：
chemiluminescence; polysiloxane; polyvinyl alcohol; solid support;
D O I：
10.1023/A:1020675815637
中图分类号：
Q81 [生物工程学（生物技术）]; Q93 [微生物学];
学科分类号：
071005 ; 0836 ; 090102 ; 100705 ;
摘要：
A polysiloxane and polyvinyl alcohol interpenetrating polymer network was synthesised and its ferromagnetic derivative was used as solid support for antigen covalent immobilisation in chemiluminescent assays. Only 0.625 mug of either Trypanosoma cruzi or Schistosoma mansoni antigens immobilized onto the magnetic particles (2.5 mg) were sufficient to detect the correspondent human IgG within a nanogram scale.
引用
收藏
页码：1705 / 1708
页数：4
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