DNA pooling: A tool for large-scale association studies

被引：456
作者：
Sham, P
Bader, JS
Craig, I
O'Donovan, M
Owen, M
机构：
[1] Kings Coll London, Inst Psychiat, London SE5 8AF, England
[2] CuraGen Corp, Dept Bioinformat, New Haven, CT 06511 USA
[3] Cardiff Univ, Dept Psychol Med, Cardiff CF14 4XN, S Glam, Wales
来源：
NATURE REVIEWS GENETICS | 2002年 / 3卷 / 11期
基金：
英国惠康基金;
关键词：
D O I：
10.1038/nrg930
中图分类号：
Q3 [遗传学];
学科分类号：
071007 ; 090102 ;
摘要：
DNA pooling is a practical way to reduce the cost of large-scale association studies to identify susceptibility loci for common diseases. Pooling allows allele frequencies in groups of individuals to be measured using far fewer PCR reactions and genotyping assays than are used when genotyping individuals. Here, we discuss recent developments in quantitative genotyping assays and in the design and analysis of pooling studies. Sophisticated pooling designs are being developed that can take account of hidden population stratification, confounders and inter-loci interactions, and that allow the analysis of haplotypes.
引用
收藏
页码：862 / 871
页数：10
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