Isolation of Mycobacterium bovis from brushtail possums with non-visible lesions

被引:8
作者
de Lisle, G. W. [1 ]
Yates, G. F. [1 ]
Coleman, J. D. [2 ]
机构
[1] AgResearch, Natl Ctr Biosecur & Infect Dis, Upper Hutt 5140, New Zealand
[2] Landcare Res, Lincoln, New Zealand
关键词
Mycobacterium bovis; brushtail possum; bacterial culture; wildlife surveillance; NEW-ZEALAND; FERRETS;
D O I
10.1080/00480169.2009.36905
中图分类号
S85 [动物医学(兽医学)];
学科分类号
0906 ;
摘要
AIM: To determine the prevalence of Mycobacterium bovis infection in brushtail possums (Trichosurus vulpecula) that did not have macroscopic lesions of bovine tuberculosis, and to evaluate culture of pooled tissues from multiple possums as a method for determining the M. bovis-infection status of wildlife populations in New Zealand. METHODS: Pools of selected tissues were collected from possums from four different populations known to be infected with M. bovis. Tissue pools from individual animals, and combined pools from multiple animals, were cultured for M. bovis. RESULTS: In the four populations investigated, the prevalence of possums with macroscopic lesions confirmed by culture to be infected with M. bovis ranged from 1 to 19 (mean 31/283; 10.9)%. The prevalence of possums with non-visible lesions that were culture positive for M. bovis in the same populations ranged from 4 to 10 (mean 24/283; 8.5)%. The mean of the log(10) cfu of M. bovis of the macroscopic lesions and of the culture-positive samples that did not have visible lesions was 3.85 (SE 0.26) and 1.46 (SE 0.26) log(10) cfu, respectively (p<0.01). Mycobacterium bovis was cultured from pools of 30-50 animals in the four populations studied. CONCLUSIONS: The finding of M. bovis infection in possums with non-visible lesions identified a potential deficiency of declaring possum populations free of M. bovis on the basis of absence of macroscopic lesions. The culturing of pools of selected tissues from multiple animals without visible lesions can be used to reduce laboratory costs of possum surveys without a major reduction in the ability to detect M. bovis infection.
引用
收藏
页码:221 / 224
页数:4
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