Adapting atomic force microscopy for cell biology

被引：92

作者：

Lehenkari, PP

Charras, GT

Nykänen, A

Horton, MA

机构：

[1] UCL, Rayne Inst, Bone & Mineral Ctr, Dept Med, London WC1E 6JJ, England

[2] Univ Oulu, Fac Med, Tech Serv Unit, Oulu, Finland

来源：

ULTRAMICROSCOPY | 2000年 / 82卷 / 1-4期

基金：

英国惠康基金;

关键词：

AFM; osteoclast; integrin;

D O I：

10.1016/S0304-3991(99)00138-2

中图分类号：

TH742 [显微镜];

学科分类号：

摘要：

We present details of our AFM modifications to produce an adaptable imaging system for the cell biologist. We have designed and validated a new inverted microscope interface and a scan head with increased Z-range, based upon the TopoMetrix Explorer AFM. We have utilised these changes, together with home-made glass ball cantilevers, to obtain topographical information over cells with large Z-dimension (over 15 mu m high), and mapped calcitonin-calcitonin receptor binding forces in living bone cells. We conclude that modified AFM can be used to evaluate intermolecular events in living cells and that this approach will ensure general application to the study of receptor-ligand interactions under truly physiological conditions. (C) 2000 Elsevier Science B.V. All rights reserved.

引用

页码：289 / 295

页数：7