Immunoassay by detecting enhanced resonance light scattering signals of immunocomplex using a common spectrofluorometer

被引：18
作者：
Zhao, Hua Wen
Huang, Cheng Zhi [1 ]
Li, Yuan Fang
机构：
[1] SW Univ, Lab Mol Chem & Chem Biol, Coll Chem & Chem Engn, Chongqing 400715, Peoples R China
[2] Third Mil Med Univ, Dept Chem, Chongqing 400038, Peoples R China
来源：
TALANTA | 2006年 / 70卷 / 03期
基金：
中国国家自然科学基金;
关键词：
resonance light scattering (RLS); immunocomplex; antibody (Ab); antigen (Ag); Gaussian distribution;
D O I：
10.1016/j.talanta.2006.01.019
中图分类号：
O65 [分析化学];
学科分类号：
070302 ; 081704 ;
摘要：
A sensitive, highly specific immunoassay method has been developed by measuring the enhanced resonance light scattering (RLS) signals of immunoreactions with simultaneously scanning both the excitation and the emission monochromators of a common spectrofluorometer. For a given content of antibody (Ab), the RLS signals of an immunoreaction follow Gaussian distribution with antigen (Ag) concentration. The central position of the Gaussian curve represents the concentration of given Ab, and the half bandwidth has proved to be a characteristic constant of a given Ab-Ag immunoreaction. With the RLS signals, the limit of detection for human immunoglobulin G (HIgG) in serum samples could reach 10 ng ml(-1), and the concentration of HIgG in blood serum samples could be detected with the recovery of 90.2-107.7% and R.S.D. of 0.8-2.7%. The results of determination for three human serum samples are identical to those obtained by immunoturbidimetry. (c) 2006 Published by Elsevier B.V.
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收藏
页码：609 / 614
页数：6
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